High sensitive and broad-range detection of cortisol in human saliva using a trap lateral flow immunoassay (trapLFI) sensor

The Analyst
Hyun-Kyung OhMin-Gon Kim

Abstract

Cortisol, a steroid hormone, is a main biomarker of psychological stress. Early diagnosis and proper treatment of such stress is crucial to prevent the excessive secretion of cortisol. However, cortisol has a low molecular weight and cannot provide sufficient recognition sites for sandwich immunoreaction; it has previously been measured using a competitive immunoassay instead of a general sandwich immunoassay. The disadvantage of this approach is that quantitative measurements are limited because of the narrow measurable range that is key for biosensors. To overcome this limitation, we propose a new detection platform that enables small molecules such as cortisol to be quantified with high detection sensitivity. A trap lateral flow immunoassay (trapLFI) sensor has deletion and detection zones instead of the test and control zones in general lateral flow immunoassay (LFI) sensors. The conjugates used to minimize possible detection targets at low concentration are gold nanoparticles that include an antibody against cortisol and an enzyme for signal generation. Target-bound conjugates are captured in the detection zone, whereas conjugates not binding with targets are trapped in the deletion zone. Using this platform, enzyme-cataly...Continue Reading

References

Jun 1, 1978·Journal of Clinical Pathology·A VollerD E Bidwell
Oct 29, 2003·Journal of Immunological Methods·Wingman LeungReinhard Renneberg
Nov 6, 2007·The British Journal of Psychiatry : the Journal of Mental Science·Marie-Louise MeewisseMiranda Olff
Dec 20, 2008·Psychoneuroendocrinology·Dirk H HellhammerBrigitte M Kudielka
Aug 19, 2010·Croatian Medical Journal·Lorraine L PerrettJudy A Stack
Nov 9, 2010·Analytica Chimica Acta·Seema NaraTulsidas G Shrivastav
Sep 4, 2012·Biosensors & Bioelectronics·Masaki YamaguchiVivek Shetty
Apr 5, 2016·The Analyst·V Cengiz ÖzalpHüseyin A Öktem

❮ Previous
Next ❯

Methods Mentioned

BETA
blood draw
enzyme-linked immunosorbent assay
ELISA

Software Mentioned

Image Lab

Related Concepts

Related Feeds

Biosensors for Cancer Detection

Biosensors are devices that are designed to detect a specific biological analyte by essentially converting a biological entity (ie, protein, DNA, RNA) into an electrical signal that can be detected and analyzed. The use of biosensors in cancer detection and monitoring holds vast potential. Biosensors can be designed to detect emerging cancer biomarkers and to determine drug effectiveness at various target sites. Biosensor technology has the potential to provide fast and accurate detection, reliable imaging of cancer cells, and monitoring of angiogenesis and cancer metastasis, and the ability to determine the effectiveness of anticancer chemotherapy agents.