High sensitive determination of sufentanil in human plasma of parturients and neonates following patient-controlled epidural analgesia (PCEA)

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Ronald SchmidtGerd Geisslinger

Abstract

A validated method for the determination of sufentanil in human plasma using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is described. Sufentanil was extracted from human plasma with solid-phase-extraction using deuterated sufentanil, [(2)H(5)]-sufentanil, as internal standard. Sufentanil and the internal standard were determined with an API 4000 tandem mass spectrometer equipped with a Turbo-V-Source operating in positive ESI mode on an Alltima HP HILIC straight phase column. The method showed a lower limit of quantification of 0.25 pg/ml (12.5 fg on column). The applicability of the method is shown in a clinical study, in which levels of sufentanil in plasma of parturients and arterial umbilical plasma of their neonates following patient-controlled epidural analgesia (PCEA) under several regimen treatments was analyzed.

Citations

Mar 29, 2011·Analytical and Bioanalytical Chemistry·M BarrosoM López-Rivadulla
Oct 15, 2013·Paediatric Anaesthesia·Bettina N NielsenSteen W Henneberg
Apr 11, 2008·Journal of Separation Science·Hien P Nguyen, Kevin A Schug
Dec 3, 2009·Chemical & Pharmaceutical Bulletin·Sankareswaran SrimuruganChinpiao Chen
Jul 27, 2010·Journal of Separation Science·Sarah CooremanKathleen Croes

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