High sensitivity deep-UV LED-based z-cell photometric detector for capillary liquid chromatography

Analytica Chimica Acta
Yan LiMirek Macka

Abstract

A new high sensitivity deep-UV LED photometric detector with a z-type flow cell (45 nL or 180 nL) for miniaturised and portable capillary liquid chromatography (LC) was designed and fabricated to overcome sensitivity limitations due to short pathlength in on-capillary detectors. The new detector has a 10 mm geometric pathlength and uses high intensity light-emitting diodes (LED) as light sources in the deep-UV range (254 nm and 280 nm). No optical reference was necessary due to the low drift in the signal. Stray light was minimized by the use of an adjustable slit with a 0.5 mm pinhole. The direct relationship between absorbance and concentration was obtained using dichromate to evaluate the sensitivity and the linearity range of the detector. Performance of the miniaturised version was compared with that obtained from a commercial benchtop detector for capillary LC under the same conditions using the same optical z-cell. The miniaturised version exhibited a superior performance across all parameters, including 3 times higher effective pathlength, 4 times higher upper limit of detector linearity, and 2-5 times lower stray light levels. An application of the new detector was shown with the detection of l-dopa, l-tyrosine, norfen...Continue Reading

Citations

Aug 17, 2020·Journal of Chromatography. a·Lewellwyn J CoatesHans-Jürgen Wirth
Jan 17, 2021·Journal of Chromatography. a·Joshua J DavisJames P Grinias
Sep 2, 2020·Analytical Chemistry·Cemil AydoğanLuigi Mondello
Oct 23, 2019·Analytical Chemistry·Alexander S KaplitzJames P Grinias

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