High sensitivity of chemiluminescent methodology for detection of clonal CDR3 sequences in patients with acute lymphoblastic leukemia

Hematological Oncology
E LealP Barros-Núñez

Abstract

Detection of minimal residual disease (MRD) in patients with B-cell acute lymphoblastic leukemia (B-ALL) has been achieved using several radioactive labelling methodologies; however, limited information exists about the use of chemiluminescent labelling. Although many malignant disorders are related to cytogenetic alterations, there is not a consistent chromosomal translocation that could serve as a tumour marker for the monitoring of MRD. ALL are derived from B-lymphocytes in 80% of cases. In the early stages of their maturation, the immunoglobulin heavy chain genes (IgH) undergo rearrangements among their V, D, and J segments, giving rise to the Complementary Determining Regions (CDR). Among these, CDR3 is considered unique for each lymphocyte and used as a tumour-specific marker in B-ALL patients. In this study, the CDR3 was labelled with digoxigenin and used as a patient-specific probe to test its sensitivity for further detection of MRD. Fourteen pretreatment samples of bone marrow (BM) or peripheral blood (PB) from B-ALL patients were included. Tumour-specific probes were designed from each clonal product by elimination of the consensus sequences. Ten digoxigenin-labelled probes were hybridized with a mixture of their res...Continue Reading

References

Jul 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M YamadaG Rovera
Apr 14, 1983·Nature·S Tonegawa
Apr 8, 1999·Leukemia & Lymphoma·Z Estrov, M H Freedman

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Citations

Mar 7, 2006·Analytical Biochemistry·Dongrui ZhouZuhong Lu
Feb 16, 2015·Clinical Reviews in Allergy & Immunology·Ying SunM Eric Gershwin

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