High soluble endoglin levels do not induce endothelial dysfunction in mouse aorta

PloS One
Ivana NemeckovaPetr Nachtigal

Abstract

Increased levels of a soluble form of endoglin (sEng) circulating in plasma have been detected in various pathological conditions related to cardiovascular system. High concentration of sEng was also proposed to contribute to the development of endothelial dysfunction, but there is no direct evidence to support this hypothesis. Therefore, in the present work we analyzed whether high sEng levels induce endothelial dysfunction in aorta by using transgenic mice with high expression of human sEng. Transgenic mice with high expression of human sEng on CBAxC57Bl/6J background (Sol-Eng+) and age-matched transgenic littermates that do not develop high levels of human soluble endoglin (control animals in this study) on chow diet were used. As expected, male and female Sol-Eng+ transgenic mice showed higher levels of plasma concentrations of human sEng as well as increased blood arterial pressure, as compared to control animals. Functional analysis either in vivo or ex vivo in isolated aorta demonstrated that the endothelium-dependent vascular function was similar in Sol-Eng+ and control mice. In addition, Western blot analysis showed no differences between Sol-Eng+ and control mice in the protein expression levels of endoglin, endotheli...Continue Reading

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Citations

Nov 2, 2015·Atherosclerosis·Jana RathouskaPetr Nachtigal
Apr 7, 2017·Heart and Vessels·Jana RathouskaPetr Nachtigal
Nov 18, 2017·PloS One·Laura Ruiz-RemolinaAlicia Rodríguez-Barbero
Aug 23, 2019·Proceedings of the National Academy of Sciences of the United States of America·Aleksandra LaweraWei Li
Feb 13, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Matej VicenPetr Nachtigal
May 30, 2020·PloS One·Barbora VitverovaPetr Nachtigal
Jan 21, 2020·Journal of Cellular and Molecular Medicine·Ivana MarkováLibor Vítek
Dec 31, 2020·International Journal of Molecular Sciences·Lucía Pérez-RoqueMiguel Pericacho
Nov 14, 2020·Cellular and Molecular Life Sciences : CMLS·Matej VicenPetr Nachtigal

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Methods Mentioned

BETA
transgenic
ELISA
electrophoresis
X-Ray

Software Mentioned

Sciences
GraphPad
NIS
Data
GraphPad Prism
Elements

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