High spatial and temporal resolution wide-field imaging of neuron activity using quantum NV-diamond.

Scientific Reports
Liam T HallLloyd C L Hollenberg

Abstract

A quantitative understanding of the dynamics of biological neural networks is fundamental to gaining insight into information processing in the brain. While techniques exist to measure spatial or temporal properties of these networks, it remains a significant challenge to resolve the neural dynamics with subcellular spatial resolution. In this work we consider a fundamentally new form of wide-field imaging for neuronal networks based on the nanoscale magnetic field sensing properties of optically active spins in a diamond substrate. We analyse the sensitivity of the system to the magnetic field generated by an axon transmembrane potential and confirm these predictions experimentally using electronically-generated neuron signals. By numerical simulation of the time dependent transmembrane potential of a morphologically reconstructed hippocampal CA1 pyramidal neuron, we show that the imaging system is capable of imaging planar neuron activity non-invasively at millisecond temporal resolution and micron spatial resolution over wide-fields.

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Citations

Aug 8, 2012·Proceedings of the National Academy of Sciences of the United States of America·Viva R HorowitzDavid D Awschalom
Mar 15, 2016·Scientific Reports·David A SimpsonLloyd C L Hollenberg
Nov 5, 2013·Neuron·Karl Deisseroth, Mark J Schnitzer
May 31, 2014·Current Opinion in Chemical Biology·Gopalakrishnan BalasubramanianDe-Wen Duan
Dec 20, 2016·Scientific Reports·Francesca BarbieriGilles Ouanounou
Jul 17, 2014·Scientific Reports·I V FedotovA M Zheltikov
Mar 12, 2017·Progress in Nuclear Magnetic Resonance Spectroscopy·Dieter Suter, Fedor Jelezko
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Feb 15, 2018·Optics Letters·Xiang-Dong ChenFang-Wen Sun
Apr 10, 2019·Scientific Reports·Christian OsterkampFedor Jelezko
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May 27, 2021·ACS Sensors·Tongtong ZhangZhiqin Chu
Jun 4, 2021·ACS Central Science·Chung-Jui YuDanna E Freedman
Nov 8, 2017·ACS Nano·David A SimpsonLloyd C L Hollenberg
Aug 21, 2019·Nano Letters·Priyadharshini BalasubramanianFedor Jelezko

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