High-spatial-resolution deep tissue imaging with spectral-domain optical coherence microscopy in the 1700-nm spectral band

Journal of Biomedical Optics
Masahito YamanakaN Nishizawa

Abstract

We present three-dimensional (3-D) high-resolution spectral-domain optical coherence microscopy (SD-OCM) by using a supercontinuum (SC) fiber laser source with 300-nm spectral bandwidth (full-width at half-maximum) in the 1700-nm spectral band. By using low-coherence interferometry with SC light and a confocal detection scheme, we realized lateral and axial resolutions of 3.4 and 3.8  μm in tissue (n  =  1.38), respectively. This is, to the best of our knowledge, the highest 3-D spatial resolution reported among those of Fourier-domain optical coherence imaging techniques in the 1700-nm spectral band. In our SD-OCM, to enhance the imaging depth, a full-range method was implemented, which suppressed the formation of a coherent ghost image and allowed us to set the zero-delay position inside the samples. We demonstrated the 3-D high-resolution imaging capability of 1700-nm SD-OCM through the measurement of an interference signal from a mirror surface and imaging of a single 200-nm polystyrene bead and a pig thyroid gland. Deep tissue imaging at a depth of up to 1.8 mm was also demonstrated. This is the first demonstration of 3-D high-resolution SD-OCM in the 1700-nm spectral band.

References

Apr 21, 2003·Optics Express·R LeitgebAdolf Fercher
Apr 15, 1994·Optics Letters·J A IzattJ G Fujimoto
Mar 10, 2010·Journal of Biomedical Optics·Aaron D AguirreJames G Fujimoto
Jan 25, 2011·Biomedical Optics Express·V M KodachT G van Leeuwen
Aug 14, 2013·Optics Express·Osman O AhsenJames G Fujimoto
Sep 7, 2013·Biomedical Optics Express·Hsiang-Chieh LeeJames G Fujimoto

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging
confocal microscopy
imaging techniques
light scattering

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.