High-speed 2D and 3D fluorescence microscopy of cardiac myocytes

Optics Express
Sunil KumarChris Dunsby

Abstract

Oblique plane microscopy (OPM) is a light sheet microscopy technique that uses a single high numerical aperture microscope objective to both illuminate a tilted plane within the specimen and to obtain an image of the tilted illuminated plane. In this paper, we present a new OPM configuration that enables both the illumination and detection focal planes to be swept simultaneously and remotely through the sample volume, enabling high speed volumetric imaging. We demonstrate the high speed imaging capabilities of the system by imaging calcium dynamics in cardiac myocytes in 2D at 926 frames per second and in 3D at 21 volumes per second. In the future, higher frame rate CCD cameras will enable volumetric imaging at much greater rates, leading to new capabilities to study dynamic events in cells at high speeds in two and three dimensions.

References

Jan 1, 1996·The American Journal of Physiology·H ChengM B Cannell
Aug 1, 1996·Cell Calcium·H ChengW Jonathan Lederer
Dec 22, 1999·The Journal of Physiology·V Lukyanenko, S Gyorke
Mar 29, 2000·Biophysical Journal·G H Patterson, D W Piston
Jan 11, 2007·Journal of Biomedical Optics·Ralf WolleschenskyMichael Kempe
Apr 13, 2007·Cell Calcium·Ernst Niggli, Natalia Shirokova
Jul 17, 2007·Optics Letters·Edward J BotcherbyTony Wilson
Dec 10, 2008·Optics Express·C Dunsby
Apr 4, 2009·Proceedings of the National Academy of Sciences of the United States of America·Alexander R LyonJulia Gorelik
May 19, 2009·Optics Letters·Edward J BotcherbyTony Wilson
May 26, 2009·Development·Jan Huisken, Didier Y R Stainier
May 19, 2010·Proceedings of the National Academy of Sciences of the United States of America·Naoya TakahashiYuji Ikegaya
Dec 15, 2010·Circulation Research·Didier X P BrochetW Jonathan Lederer

Citations

Nov 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·David R SmithRandy A Bartels
Dec 2, 2014·Scientific Reports·Tongcang LiXiang Zhang
Dec 29, 2017·Journal of Biophotonics·Younghoon ShinHyuk-Sang Kwon
May 2, 2018·Annual Review of Neuroscience·Siegfried Weisenburger, Alipasha Vaziri
Oct 10, 2013·Optics Express·Florian O FahrbachJan Huisken
May 9, 2019·Biomedical Optics Express·Aleks PonjavicDavid Klenerman
May 1, 2020·Light, Science & Applications·Pablo Loza-Alvarez
Jul 9, 2020·Biomedical Optics Express·Manish Kumar, Yevgenia Kozorovitskiy
Jul 9, 2020·Biomedical Optics Express·Michele GintoliAlexander D Corbett
Feb 28, 2014·Biomedical Optics Express·Joey M JabbourKristen C Maitland
Jan 8, 2021·Biomedical Optics Express·Hugh SparksChris Dunsby
Apr 21, 2021·Light, Science & Applications·Pablo Loza-Alvarez

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.