Oblique plane microscopy (OPM) is a light sheet microscopy technique that uses a single high numerical aperture microscope objective to both illuminate a tilted plane within the specimen and to obtain an image of the tilted illuminated plane. In this paper, we present a new OPM configuration that enables both the illumination and detection focal planes to be swept simultaneously and remotely through the sample volume, enabling high speed volumetric imaging. We demonstrate the high speed imaging capabilities of the system by imaging calcium dynamics in cardiac myocytes in 2D at 926 frames per second and in 3D at 21 volumes per second. In the future, higher frame rate CCD cameras will enable volumetric imaging at much greater rates, leading to new capabilities to study dynamic events in cells at high speeds in two and three dimensions.
Orthogonal-plane fluorescence optical sectioning: three-dimensional imaging of macroscopic biological specimens
Fast three-dimensional fluorescence imaging of activity in neural populations by objective-coupled planar illumination microscopy
Axial stretch of rat single ventricular cardiomyocytes causes an acute and transient increase in Ca2+ spark rate
Loss of T-tubules and other changes to surface topography in ventricular myocytes from failing human and rat heart
Quantifying distortions in two-photon remote focussing microscope images using a volumetric calibration specimen
Swept confocally-aligned planar excitation (SCAPE) microscopy for high speed volumetric imaging of behaving organisms
Time-lapse 3-D measurements of a glucose biosensor in multicellular spheroids by light sheet fluorescence microscopy in commercial 96-well plates
A Guide to Emerging Technologies for Large-Scale and Whole-Brain Optical Imaging of Neuronal Activity
High speed sCMOS-based oblique plane microscopy applied to the study of calcium dynamics in cardiac myocytes
Fast measurement of sarcomere length and cell orientation in Langendorff-perfused hearts using remote focusing microscopy
Development a flexible light-sheet fluorescence microscope for high-speed 3D imaging of calcium dynamics and 3D imaging of cellular microstructure.
Oblique scanning laser microscopy for simultaneously volumetric structural and molecular imaging using only one raster scan
Integrated one- and two-photon scanned oblique plane illumination (SOPi) microscopy for rapid volumetric imaging
Performance tradeoffs for single- and dual-objective open-top light-sheet microscope designs: a simulation-based analysis
A versatile oblique plane microscope for large-scale and high-resolution imaging of subcellular dynamics.
Cell Imaging in CNS
Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.