High-speed biomarker identification utilizing porous silicon nanovial arrays and MALDI-TOF mass spectrometry

Electrophoresis
D FinnskogThomas Laurell

Abstract

Speed and accuracy are crucial prerequisites in the application of proteomic methods to clinical medicine. We describe a microfluidic-based nanovial array for rapid proteolytic processing linked to MALDI-TOF MS. This microscale format consumes only minute amounts of sample, and it is compatible with rapid bioanalytical protocols and high-sensitivity readouts. Arrays of vials (300 microm in diameter and 25 microm deep), isotropically etched in silicon wafers were electrochemically porosified. Automated picoliter microdispensing was employed for precise fluid handling in the microarray format. Vials were prefilled with trypsin solution, which was allowed to dry. Porosified and nonporosified nanovials were compared for trypsin digestion and subsequent MS identification of three model proteins: lysozyme, alcohol dehydrogenase, and serum albumin at levels of 100 and 20 fmol. In an effort to assess the rapid digestion platform in a context of putative clinical applications, two prostate cancer biomarkers, prostate-specific antigen (PSA) and human glandular kallikrein 2 (hK2), were digested at levels of 100 fmol (PSA), 20 fmol (PSA) and 8 fmol (hK2). All biomarker digestions were completed in less than 30 s, with successful MS identif...Continue Reading

References

Jun 1, 1991·Trends in Biotechnology·M Wahlgren, T Arnebrant
May 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H Lilja, A Lundwall
Aug 1, 1987·The Journal of Clinical Investigation·H LiljaC B Laurell
Sep 23, 1997·Biochemical and Biophysical Research Communications·J LövgrenH Lilja
Feb 3, 2000·Analytical Chemistry·C EricsonS Hjertén
Apr 29, 2000·Electrophoresis·K Gevaert, J Vandekerckhove
Sep 21, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·E Litborn, J Roeraade
Apr 11, 2001·Proceedings of the National Academy of Sciences of the United States of America·J J ThomasG Siuzdak
Jan 29, 2002·Analytical Chemistry·Hanbin MaoPaul S Cremer
Mar 14, 2002·Electrophoresis·Joseph Wang
Dec 13, 2003·Analytical Chemistry·Anton RessineThomas Laurell

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Citations

Nov 7, 2006·Analytical and Bioanalytical Chemistry·Chensong PanHanfa Zou
Aug 25, 2010·Analytical Chemistry·Basri GulbakanWeihong Tan
Oct 31, 2006·Omics : a Journal of Integrative Biology·Christer Wingren, Carl A K Borrebaeck
Jan 1, 2010·International Journal of Molecular Sciences·Mohan Natesan, Robert G Ulrich
Jan 15, 2014·Omics : a Journal of Integrative Biology·Firas H KobeissyKevin Wang
Apr 5, 2011·Annual Review of Analytical Chemistry·Jason S Kuo, Daniel T Chiu
Apr 24, 2009·Expert Review of Proteomics·Oda StoevesandtMingyue He
Jun 8, 2007·Expert Review of Proteomics·William C S Cho, Christopher H K Cheng
May 22, 2009·Journal of Proteomics·Carl A K Borrebaeck, Christer Wingren
Jan 12, 2008·Current Opinion in Biotechnology·Christer Wingren, Carl Ak Borrebaeck
Apr 4, 2007·Mass Spectrometry Reviews·Xuewu ZhangFeng Chen
Jan 3, 2007·Journal of Mass Spectrometry : JMS
Jan 22, 2011·Mass Spectrometry Reviews·Pawel L UrbanRenato Zenobi
Aug 28, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Kerstin JäråsThomas Laurell
Nov 30, 2014·Applied Biochemistry and Biotechnology·Anna Smolira, Joanna Wessely-Szponder
Mar 13, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·SangWook LeeSoyoun Kim

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