High-speed Fourier ptychographic microscopy based on programmable annular illuminations

Scientific Reports
Jiasong SunQian Chen

Abstract

High-throughput quantitative phase imaging (QPI) is essential to cellular phenotypes characterization as it allows high-content cell analysis and avoids adverse effects of staining reagents on cellular viability and cell signaling. Among different approaches, Fourier ptychographic microscopy (FPM) is probably the most promising technique to realize high-throughput QPI by synthesizing a wide-field, high-resolution complex image from multiple angle-variably illuminated, low-resolution images. However, the large dataset requirement in conventional FPM significantly limits its imaging speed, resulting in low temporal throughput. Moreover, the underlying theoretical mechanism as well as optimum illumination scheme for high-accuracy phase imaging in FPM remains unclear. Herein, we report a high-speed FPM technique based on programmable annular illuminations (AIFPM). The optical-transfer-function (OTF) analysis of FPM reveals that the low-frequency phase information can only be correctly recovered if the LEDs are precisely located at the edge of the objective numerical aperture (NA) in the frequency space. By using only 4 low-resolution images corresponding to 4 tilted illuminations matching a 10×, 0.4 NA objective, we present the hig...Continue Reading

References

May 14, 2004·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Colin J R Sheppard
Nov 16, 2006·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Vicente MicoJavier García
Dec 13, 2006·Physical Review Letters·Sergey A AlexandrovDavid D Sampson
Jan 3, 2007·Developmental Cell·Estelle Glory, Robert F Murphy
Jul 3, 2007·British Journal of Pharmacology·V Starkuviene, R Pepperkok
Dec 12, 2007·Optics Letters·E CucheC Depeursinge
Dec 19, 2007·Optics Letters·A BartyA Roberts
Jun 12, 2008·Optics Express·Manuel Guizar-Sicairos, James R Fienup
Feb 7, 2009·Methods in Cell Biology·Gabriel Popescu
Feb 10, 2009·Ultramicroscopy·Pierre ThibaultFranz Pfeiffer
Jun 23, 2009·Ultramicroscopy·Andrew M Maiden, John M Rodenburg
Jul 19, 2011·Optics Letters·Shan Shan KouColin J R Sheppard
Oct 5, 2011·Proceedings of the National Academy of Sciences of the United States of America·Guoan ZhengChanghuei Yang
Feb 8, 2013·Nature·Pierre Thibault, Andreas Menzel
Oct 10, 2013·Optics Letters·Chao ZuoAnand Asundi
Dec 11, 2013·Optics Letters·Xiaoze OuGuoan Zheng
Feb 12, 2014·Optics Express·Zichao BianGuoan Zheng
Mar 26, 2014·Optics Express·Xiaoze OuChanghuei Yang
Jun 19, 2014·Biomedical Optics Express·Siyuan DongGuoan Zheng
Jul 30, 2014·Biomedical Optics Express·Lei TianLaura Waller
Sep 23, 2014·Nature Photonics·Guoan ZhengChanghuei Yang
Dec 10, 2014·Optics Letters·Liheng BianQionghai Dai
May 15, 2015·Optics Express·Lei Tian, Laura Waller
Jul 26, 2016·Biomedical Optics Express·Jiasong SunChao Zuo
Oct 31, 2017·Biomedical Optics Express·Jiaji LiChao Zuo

❮ Previous
Next ❯

Citations

Feb 6, 2020·Biomedical Optics Express·Remy ClaveauDelmiro Fernandez-Reyes
Nov 25, 2018·Optics Express·Samuel McDermott, Andrew Maiden
Oct 28, 2019·Biomedical Optics Express·Nicholas AnthonyBrian Abbey
Apr 1, 2020·Optics Express·Pavan Chandra KondaRoarke Horstmeyer

❮ Previous
Next ❯

Methods Mentioned

BETA
imaging technique

Software Mentioned

CameraLink
MATLAB
AIFPM

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Advanced Imaging of Cellular Signaling

Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.