Genetically encoded voltage indicators (GEVIs) are a promising technology for fluorescence readout of millisecond-scale neuronal dynamics. Previous GEVIs had insufficient signaling speed and dynamic range to resolve action potentials in live animals. We coupled fast voltage-sensing domains from a rhodopsin protein to bright fluorophores through resonance energy transfer. The resulting GEVIs are sufficiently bright and fast to report neuronal action potentials and membrane voltage dynamics in awake mice and flies, resolving fast spike trains with 0.2-millisecond timing precision at spike detection error rates orders of magnitude better than previous GEVIs. In vivo imaging revealed sensory-evoked responses, including somatic spiking, dendritic dynamics, and intracellular voltage propagation. These results empower in vivo optical studies of neuronal electrophysiology and coding and motivate further advancements in high-speed microscopy.
Engineering of a genetically encodable fluorescent voltage sensor exploiting fast Ci-VSP voltage-sensing movements
New rabies virus variants for monitoring and manipulating activity and gene expression in defined neural circuits
Single action potentials and subthreshold electrical events imaged in neurons with a fluorescent protein voltage probe
High-fidelity optical reporting of neuronal electrical activity with an ultrafast fluorescent voltage sensor
Complementary contributions of spike timing and spike rate to perceptual decisions in rat S1 and S2 cortex
All-optical control of cardiac excitation: combined high-resolution optogenetic actuation and optical mapping
Adult Born Olfactory Bulb Dopaminergic Interneurons: Molecular Determinants and Experience-Dependent Plasticity
Imaging voltage in zebrafish as a route to characterizing a vertebrate functional connectome: promises and pitfalls of genetically encoded indicators
Electrical Identification and Selective Microstimulation of Neuronal Compartments Based on Features of Extracellular Action Potentials
FHF-independent conduction of action potentials along the leak-resistant cerebellar granule cell axon
The Oscillating Stimulus Transporter Assay, OSTA: Quantitative Functional Imaging of Transporter Protein Activity in Time and Frequency Domains
Painting with Rainbows: Patterning Light in Space, Time, and Wavelength for Multiphoton Optogenetic Sensing and Control
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Graphics Processing Unit-Accelerated Code for Computing Second-Order Wiener Kernels and Spike-Triggered Covariance
Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission
Glutamatergic synapses are structurally and biochemically complex because of multiple plasticity processes: long-term potentiation, long-term depression, short-term potentiation and scaling
Transgenic Strategies for Sparse but Strong Expression of Genetically Encoded Voltage and Calcium Indicators
Fast two-photon imaging of subcellular voltage dynamics in neuronal tissue with genetically encoded indicators
Insertion of the voltage-sensitive domain into circularly permuted red fluorescent protein as a design for genetically encoded voltage sensor
Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons.
Single-Neuron Level One-Photon Voltage Imaging With Sparsely Targeted Genetically Encoded Voltage Indicators
Focusing light inside live tissue using reversibly switchable bacterial phytochrome as a genetically encoded photochromic guide star
Novel Genetically Encoded Bright Positive Calcium Indicator NCaMP7 Based on the mNeonGreen Fluorescent Protein
Exploring concomitant neuroimaging and genetic alterations in patients with and patients without auditory verbal hallucinations: A pilot study and mini review
Wide-field optical mapping of neural activity and brain haemodynamics: considerations and novel approaches
Syringe-injectable mesh electronics integrate seamlessly with minimal chronic immune response in the brain
All-optical imaging and manipulation of whole-brain neuronal activities in behaving larval zebrafish
High-speed interferometric imaging reveals dynamics of neuronal deformation during the action potential
Spectro-temporal encoded multiphoton microscopy and fluorescence lifetime imaging at kilohertz frame-rates
A note on retrograde gene transfer efficiency and inflammatory response of lentiviral vectors pseudotyped with FuG-E vs. FuG-B2 glycoproteins
Using Genetically Encoded Voltage Indicators (GEVIs) to Study the Input-Output Transformation of the Mammalian Olfactory Bulb
Absorption and Emission Spectroscopic Investigation of the Thermal Dynamics of the Archaerhodopsin 3 Based Fluorescent Voltage Sensor Archon2.
Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging
High frequency, real-time neurochemical and neuropharmacological measurements in situ in the living body
Recent progress in optical voltage-sensor technology and applications to cardiac research: from single cells to whole hearts.
Molecular Tuning of the Axonal Mitochondrial Ca2+ Uniporter Ensures Metabolic Flexibility of Neurotransmission.
Engineering genetically encoded fluorescent indicators for imaging of neuronal activity: Progress and prospects
Recent advances in the use of genetically encodable optical tools to elicit and monitor signaling events.
Deconvolution of Voltage Sensor Time Series and Electro-diffusion Modeling Reveal the Role of Spine Geometry in Controlling Synaptic Strength
Novel Deconvolution Kernel for Extended Depth-of-Field Microscopy with a High-Speed Deformable Mirror
Pre-resonance stimulated Raman scattering spectroscopy and imaging of membrane potential using near-infrared rhodopsins
Advanced Imaging of Cellular Signaling
Cell signaling is a vital mechanism for communication within cells and outside with the environment. Several different signaling pathways have been found and advanced imaging techniques are being developed to visualize the molecules involved in these signaling pathways. Find the latest research in advanced imaging of cellular signaling here.
BRAIN Initiative Cell Census Network (BICCN)
The BRAIN Initiative Cell Census Network aims to identify and provide experimental access to the different brain cell types to determine their roles in health and disease. Discover the latest research from researchers in the BRAIN Initiative Cell Census Network here.
Here is the latest research on barrel cortex, a region of somatosensory and motor corticies in the brain, which are used by animals that rely on whiskers for world exploration.