High Surface Area Electrodes Generated via Electrochemical Roughening Improve the Signaling of Electrochemical Aptamer-Based Biosensors

Analytical Chemistry
Netzahualcóyotl Arroyo-CurrásKevin W Plaxco

Abstract

The electrochemical, aptamer-based (E-AB) sensor platform provides a modular approach to the continuous, real-time measurement of specific molecular targets (irrespective of their chemical reactivity) in situ in the living body. To achieve this, however, requires the fabrication of sensors small enough to insert into a vein, which, for the rat animal model we employ, entails devices less than 200 μm in diameter. The limited surface area of these small devices leads, in turn, to low faradaic currents and poor signal-to-noise ratios when deployed in the complex, fluctuating environments found in vivo. In response we have developed an electrochemical roughening approach that enhances the signaling of small electrochemical sensors by increasing the microscopic surface area of gold electrodes, allowing in this case more redox-reporter-modified aptamers to be packed onto the surface, thus producing significantly improved signal-to-noise ratios. Unlike previous approaches to achieving microscopically rough gold surfaces, our method employs chronoamperometric pulsing in a 5 min etching process easily compatible with batch manufacturing. Using these high surface area electrodes, we demonstrate the ability of E-AB sensors to measure comp...Continue Reading

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Citations

Dec 28, 2019·International Journal of Molecular Sciences·Wei XuChung Chiun Liu
Feb 23, 2019·Nature Communications·Shaolin LiangJulian A Tanner
Jul 31, 2019·Translational Research : the Journal of Laboratory and Clinical Medicine·Karen ScidaBrian G Jamieson
Aug 25, 2020·Journal of Pharmaceutical Analysis·Sumin BianMohamad Sawan
Sep 23, 2020·Analytical Chemistry·Alex M DownsPhilippe Dauphin-Ducharme
Apr 3, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jacopo MovilliJurriaan Huskens
Aug 30, 2019·Analytical Chemistry·Samuel D CurtisNetzahualcóyotl Arroyo-Currás
Jul 4, 2020·ACS Sensors·Claudio ParoloKevin W Plaxco
Jan 8, 2022·Science Advances·Ji-Won SeoHyongsok T Soh

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