High survival of mouse oocytes/embryos after vitrification without permeating cryoprotectants followed by ultra-rapid warming with an IR laser pulse

Scientific Reports
Bo Jin, Peter Mazur

Abstract

Vitrification is now the main route to the cryopreservation of human and animal oocytes and preimplantation embryos. A central belief is that for success, the cells must be placed in very high concentrations of cryoprotective solutes and must be cooled extremely rapidly. We have shown recently that these beliefs are incorrect. Over 90% of mouse oocytes and embryos survive being cooled relatively slowly even in solutions containing only 1/3(rd) the normal solute concentrations, provided that they are warmed ultra-rapidly at 10(7)°C/min by a laser pulse. Nearly all vitrification solutions contain both permeating and non-permeating solutes, and an important question is whether the former protect because they permeate the cells and promote intracellular vitrification (as is almost universally believed), or because they osmotically withdraw a large fraction of intracellular water prior to cooling. The answer for the mouse system is clearly the latter. When oocytes or embryos are placed in 1 molal concentrations of the impermeable solute sucrose, they osmotically lose ~85% of their cellular water in less than 2 minutes. If the cells are then cooled rapidly to -196°C, nearly 90% remain viable after warming, again provided that the war...Continue Reading

References

May 1, 1995·Biophysical Journal·Y LiuB J Tromberg
Jun 25, 2005·Cryobiology·Peter MazurKeisuke Edashige
Apr 3, 2008·Journal of the Royal Society, Interface·Hu Zhang, Kuo-Kang Liu

❮ Previous
Next ❯

Citations

Sep 20, 2015·Cell and Tissue Banking·Tatyana M GurinaGalyna A Bozhok
Sep 27, 2018·Biopreservation and Biobanking·Sergei AmstislavskyPierre Comizzoli
Sep 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Blasa Carmen PereiraManuel Hidalgo
Sep 3, 2020·Advanced Healthcare Materials·Zonghu HanJohn C Bischof
Jun 6, 2018·Annual Review of Biomedical Engineering·John C Bischof, Kenneth R Diller
Feb 1, 2015·Reproduction, Fertility, and Development·George E Seidel
Nov 26, 2016·Reproduction in Domestic Animals = Zuchthygiene·L Fernandez-Gonzalez, K Jewgenow
Oct 26, 2018·Scientific Reports·Jonathan DalyMary Hagedorn
Sep 12, 2020·Emerging Topics in Life Sciences·Kylie BaldwinNicky Hudson
Feb 6, 2017·Biotechnology Advances·Gang Zhao, Jianping Fu
Mar 23, 2021·Advanced Science·Tie Chang, Gang Zhao
Jun 3, 2021·Animals : an Open Access Journal From MDPI·Lorena Fernandez-GonzalezKatarina Jewgenow
Aug 18, 2021·Cryobiology·Akalabya Bissoyi, Ido Braslavsky
Sep 22, 2021·Journal of Assisted Reproduction and Genetics·Hunter GiuncoDara S Berger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.