High temperature in combination with UV irradiation enhances horizontal transfer of stx2 gene from E. coli O157:H7 to non-pathogenic E. coli.

PloS One
Wan-Fu YueMei-Jun Zhu

Abstract

Shiga toxin (stx) genes have been transferred to numerous bacteria, one of which is E. coli O157:H7. It is a common belief that stx gene is transferred by bacteriophages, because stx genes are located on lambdoid prophages in the E. coli O157:H7 genome. Both E. coli O157:H7 and non-pathogenic E. coli are highly enriched in cattle feedlots. We hypothesized that strong UV radiation in combination with high temperature accelerates stx gene transfer into non-pathogenic E. coli in feedlots. E. coli O157:H7 EDL933 strain were subjected to different UV irradiation (0 or 0.5 kJ/m(2)) combination with different temperature (22, 28, 30, 32, and 37 °C) treatments, and the activation of lambdoid prophages was analyzed by plaque forming unit while induction of Stx2 prophages was quantified by quantitative real-time PCR. Data showed that lambdoid prophages in E. coli O157:H7, including phages carrying stx2, were activated under UV radiation, a process enhanced by elevated temperature. Consistently, western blotting analysis indicated that the production of Shiga toxin 2 was also dramatically increased by UV irradiation and high temperature. In situ colony hybridization screening indicated that these activated Stx2 prophages were capable of c...Continue Reading

References

May 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D G EnnisD W Mount
Oct 1, 1987·Microbial Pathogenesis·H DanbaraM Yoshikawa
Oct 27, 1997·Journal of Bacteriology·M ObuchowskiA B Oppenheim
Jul 21, 1998·Molecular & General Genetics : MGG·M GabigG Wegrzyn
Mar 22, 2000·Proceedings of the National Academy of Sciences of the United States of America·R O ElderW W Laegreid
Oct 19, 2001·Pediatric Nephrology : Journal of the International Pediatric Nephrology Association·P E Ray, X H Liu
Jun 10, 2006·The Journal of Physiology·Mei J ZhuMin Du
Oct 24, 2006·Applied and Environmental Microbiology·Michael W SandersonM J Alam
Dec 26, 2006·Journal of Food Protection·M J Alam, L Zurek
Dec 26, 2006·Applied and Environmental Microbiology·Ji Youn LimCarolyn J Hovde
Oct 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Yoshitoshi OguraTetsuya Hayashi
Jan 15, 2010·FEMS Immunology and Medical Microbiology·Joanna M LośGrzegorz Wegrzyn
Sep 17, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Mei J ZhuStephen P Ford

❮ Previous
Next ❯

Citations

Aug 22, 2012·Archives of Virology·Lidija TruncaiteRolandas Meškys
Jun 19, 2013·Journal of Applied Microbiology·B A SullivanR Karthikeyan
Dec 7, 2014·Microbiology·Alejandra Krüger, Paula M A Lucchesi
Sep 9, 2015·Frontiers in Microbiology·Jia Hu, Mei-Jun Zhu
Jun 22, 2017·Environmental Science and Pollution Research International·Jatinder P S SidhuSimon Toze
May 8, 2015·Tropical Animal Health and Production·Mohammed KamelAmr El-Sayed
Jun 7, 2018·Horticulture Research·Fang DingYongping Duan

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
Assay
ELISA

Software Mentioned

SAS
Quantity One

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.