High-threshold, Kv3-like potassium currents in magnocellular neurosecretory neurons and their role in spike repolarization

Journal of Neurophysiology
Talent ShevchenkoWilliam E Armstrong

Abstract

We identified Kv3-like high-threshold K+ currents in hypothalamic supraoptic neurons using whole cell recordings in hypothalamic slices and in acutely dissociated neurons. Tetraethylammonium (TEA)-sensitive currents (< 1 mM TEA) evoked from -50 mV were characterized by a large component that inactivated in 10-30 ms, and a smaller, persistent component that inactivated in 1-2 s. I/V relations in dissociated neurons revealed TEA-subtracted currents with a slope and voltage dependency consistent with the presence of Kv3-like channels. In slices, tests with 0.01-0.7 mM TEA produced an IC50 of 200-300 nM for both fast and persistent currents. The fast transient current was similar to currents associated with the expression of Kv3.4 subunits, given that it was sensitive to BDS-I (100 nM). The persistent TEA-sensitive current appeared similar to those attributed to Kv3.1/3.2 subunits. Although qualitatively similar, oxytocin (OT) and vasopressin (VP) neurons in slices differed in the stronger presence of persistent current in VP neurons. In both cell types, the IC50 for TEA-induced spike broadening was similar to that observed for current suppression in voltage clamp. However, TEA had a greater effect on the spike width of VP neurons ...Continue Reading

References

Sep 1, 1992·The Journal of Physiology·S H Oliet, C W Bourque
Jan 1, 1989·Methods in Cell Biology·D A AgardJ W Sedat
Aug 1, 1981·Pflügers Archiv : European journal of physiology·O P HamillF J Sigworth
Jun 15, 1996·The Journal of Physiology·S H HeinemannO Pongs
Dec 31, 1998·Journal of Neuroendocrinology·M Ludwig
Jul 22, 1999·Annals of the New York Academy of Sciences·W A CoetzeeB Rudy
Jul 22, 1999·Annals of the New York Academy of Sciences·B RudyE Vega-Saenz de Miera
Aug 17, 2001·Trends in Neurosciences·B Rudy, C J McBain
Dec 11, 2002·Journal of Neuroendocrinology·R Teruyama, W E Armstrong
Feb 20, 2003·Nature Neuroscience·Gytis BaranauskasD James Surmeier
Aug 19, 2003·The Journal of Biological Chemistry·Fernando R FernandezRay W Turner
Nov 18, 2003·Journal of Computational Neuroscience·Peter RoperWilliam Armstrong

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Citations

Oct 6, 2007·Molecular Neurobiology·Gytis Baranauskas
Mar 24, 2012·Journal of Neurophysiology·Pin LiuBruce P Bean
Nov 24, 2012·Journal of Neurophysiology·Reese ScroggsWilliam E Armstrong
Sep 24, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shuk Yin M YeungBrian Robertson
May 10, 2005·The Journal of Physiology·Ryoichi Teruyama, William E Armstrong
Apr 20, 2010·Brain Research Reviews·Alex M Thomson, William E Armstrong
Dec 22, 2004·The European Journal of Neuroscience·Ruth E BrookeJim Deuchars
Sep 15, 2017·Physiological Reviews·Leonard K Kaczmarek, Yalan Zhang
Dec 7, 2018·Journal of Neuroendocrinology·William E ArmstrongCelia D Sladek
Jul 15, 2005·Journal of Neurophysiology·Matthias GruhnRonald M Harris-Warrick
Feb 14, 2006·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Tevye J E Stachniak, Charles W Bourque
Sep 17, 2017·Nature Communications·Emmanuelle BerretJun Hee Kim
Aug 26, 2011·The Journal of Experimental Biology·Rheanna M SandWarren J Gallin

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