High-throughput analytical method for 265 pesticides and environmental contaminants in meats and poultry by fast low pressure gas chromatography and ultrahigh-performance liquid chromatography tandem mass spectrometry

Journal of Chromatography. a
Yelena Sapozhnikova

Abstract

A high throughput method for simultaneous determination of 200 pesticides and 65 environmental contaminants: polychlorinated biphenyls (PCBs), polycyclic aromatic hydrocarbons (PAHs), polybrominated diphenyl ethers (PBDEs) and other flame retardants, was developed and validated in cattle, swine and poultry muscle tissues. The method was based on sample preparation with QuEChERS extraction followed by an automated robotic sample cleanup with solid phase extraction (SPE) mini-columns using Instrument Top Sample Preparation (ITSP). The automated robotic ITSP was integrated with instrumental analysis (low pressure (LP)GC-MS/MS) to conduct sample cleanup and analysis in parallel. Another aliquot of the initial QuEChERS extract was subjected to UHPLC-MS/MS analysis, and 53 overlapping pesticides were analyzed by both LPGC- and UHPLC-MS/MS (10 min each). Fit-for-purpose LOQs<5 ng/g were achieved for ≥90% of the analytes. The method was validated in cattle, swine and chicken muscles at three spiking levels at and below the US regulatory levels of concern, and satisfactory recoveries (70-120%) and RSDs ≤ 20% were demonstrated for 219 analytes. Matrix effects were within ±20% for 78-97% of LC- and GC-amenable analytes. The validated meth...Continue Reading

References

Sep 1, 2005·Analytical and Bioanalytical Chemistry·Carmen López-BlancoJesús Simal-Gándara
Feb 17, 2015·Journal of Agricultural and Food Chemistry·Yelena Sapozhnikova, Steven J Lehotay
Jun 13, 2017·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Sara J Lupton, Heldur Hakk

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Citations

Jun 25, 2019·Water Environment Research : a Research Publication of the Water Environment Federation·Yifan WangJinkai Xue
May 1, 2020·Analytical and Bioanalytical Chemistry·Sherri B Turnipseed, Hiranthi Jayasuriya
Dec 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yongho ShinHyun Ho Noh
Oct 23, 2020·Food Science and Biotechnology·Hye Soon KangWon-Jo Choe
Feb 13, 2020·Journal of Agricultural and Food Chemistry·Virgínia Cruz FernandesValentina F Domingues

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