High-throughput and sensitive screening by ultra-performance liquid chromatography tandem mass spectrometry of diuretics and other doping agents

European Journal of Mass Spectrometry
R VenturaJ Segura

Abstract

The reliability of ultra-performance liquid chromatography coupled to tandem mass spectrometry (UPLC- MS/MS) for high throughput screening in anti-doping control has been tested. A method to screen for the presence of diuretics and other doping agents in urine has been optimised and validated. The extraction procedure consisted of an alkaline extraction (pH 9.5) with ethyl acetate and salting-out effect (sodium chloride). The extracts were analysed by UPLC-MS/MS. Analysis of 34 forbidden drugs and metabolites was achieved in a total run time of 5 min, using a C18 column (100 mm x 2.1 mm i.d., 1.7 microm particle size) and a mobile phase containing deionised water and acetonitrile with formic acid, with gradient elution at a flow-rate of 0.6 mL min(-1). Identification of the compounds was performed by multiple reaction monitoring, using electrospray ionisation in positive- or negative-ion mode. Precursor and product ions were studied for each compound and cone voltage and collision energy were optimised. Due to the different chemical structure of the compounds under study, extraction recoveries varied from less than 10% to 100% depending on the analyte. The limits of detection ranged from 50 ng mL(-1) to 200 ng mL(-1), and all t...Continue Reading

References

Jan 1, 1986·Journal of Clinical Pharmacology·R AchariJ D Hulse
Dec 6, 1996·Journal of Chromatography. B, Biomedical Applications·R Ventura, J Segura
Mar 12, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·C JiménezJ Segura
Sep 5, 2003·Rapid Communications in Mass Spectrometry : RCM·K Deventer, F T Delbeke
Jan 23, 2004·Chemical Society Reviews·Graham J Trout, Rymantas Kazlauskas
Jun 4, 2005·Journal of Mass Spectrometry : JMS·Mario ThevisWilhelm Schänzer
Feb 14, 2006·Rapid Communications in Mass Spectrometry : RCM·K DeventerF T Delbeke
Aug 21, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Lucia PolitiAldo Polettini
Mar 19, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·T KovalczukJ Hajslová

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Citations

Aug 12, 2014·Journal of Analytical Toxicology·David W Andersen, Kristian Linnet
May 25, 2010·Analytical and Bioanalytical Chemistry·C GómezR Ventura
Mar 23, 2011·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Nov 19, 2010·Bioanalysis·Pranav S ShrivastavMallika Sanyal
Jun 6, 2012·The Journal of Steroid Biochemistry and Molecular Biology·C GómezR Ventura
Mar 3, 2015·Drug Testing and Analysis·Núria MonfortRosa Ventura
Apr 17, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Georgina BalcellsRosa Ventura
Nov 7, 2015·Biomedical Chromatography : BMC·Homayon Ahmad PanahiMonireh Chabouk
Dec 22, 2010·European Journal of Mass Spectrometry·Maximiliano S Sangoi, Martin Steppe
Apr 12, 2011·Analytical and Bioanalytical Chemistry·K DeventerP Van Eenoo
Sep 22, 2020·Analytical and Bioanalytical Chemistry·Claudia BressanRosa Ventura

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