High-throughput bioconjugation for enhanced 193 nm photodissociation via droplet phase initiated ion/ion chemistry using a front-end dual spray reactor

Analytical Chemistry
Victoria C CothamJennifer S Brodbelt

Abstract

Fast online chemical derivatization of peptides with an aromatic label for enhanced 193 nm ultraviolet photodissociation (UVPD) is demonstrated using a dual electrospray reactor implemented on the front-end of a linear ion trap (LIT) mass spectrometer. The reactor facilitates the intersection of protonated peptides with a second population of chromogenic 4-formyl-1,3-benzenedisulfonic acid (FBDSA) anions to promote real-time formation of ion/ion complexes at atmospheric pressure. Subsequent collisional activation of the ion/ion intermediate results in Schiff base formation generated via reaction between a primary amine in the peptide cation and the aldehyde moiety of the FBDSA anion. Utilizing 193 nm UVPD as the subsequent activation step in the MS(3) workflow results in acquisition of greater primary sequence information relative to conventional collision induced dissociation (CID). Furthermore, Schiff-base-modified peptides exhibit on average a 20% increase in UVPD efficiency compared to their unmodified counterparts. Due to the efficiency of covalent labeling achieved with the dual spray reactor, we demonstrate that this strategy can be integrated into a high-throughput LC-MS(n) workflow for rapid derivatization of peptide m...Continue Reading

References

Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KeoughM P Lacey
Jun 15, 2006·Analytical Chemistry·Hamid Mirzaei, Fred Regnier
Dec 18, 2007·Journal of the American Society for Mass Spectrometry·Yu Xia, Scott A McLuckey
Aug 26, 2009·Journal of the American Chemical Society·Hongling Han, Scott A McLuckey
Feb 4, 2010·Journal of the American Chemical Society·Hugh I KimJ L Beauchamp
May 7, 2010·Analytical Chemistry·Wai Siang LawRenato Zenobi
Jun 29, 2010·Journal of Proteome Research·James A MadsenJennifer S Brodbelt
Oct 22, 2010·Analytical Chemistry·Lisa Vasicek, Jennifer S Brodbelt
Dec 7, 2010·Journal of the American Chemical Society·Marija Mentinova, Scott A McLuckey
Nov 17, 2011·Nature Chemical Biology·Nicholas Stephanopoulos, Matthew B Francis
Jan 31, 2012·Analytical Chemistry·Michelle R RobinsonJennifer S Brodbelt
Mar 7, 2012·Molecular & Cellular Proteomics : MCP·Lisa VasicekJennifer S Brodbelt
Mar 13, 2012·International Journal of Mass Spectrometry·John R StutzmanScott A McLuckey
May 11, 2012·Analytical Chemistry·Saša M MiladinovićYury O Tsybin
Jun 19, 2012·Journal of Mass Spectrometry : JMS·John R StutzmanScott A McLuckey
Aug 21, 2012·Angewandte Chemie·Abraham K Badu-TawiahR Graham Cooks
Oct 9, 2012·Angewandte Chemie·Thomas MüllerR Graham Cooks
Dec 5, 2012·Journal of the American Society for Mass Spectrometry·Boone M PrenticeScott A McLuckey
Dec 22, 2012·Chemical Communications : Chem Comm·Boone M Prentice, Scott A McLuckey
Nov 2, 2013·Journal of the American Society for Mass Spectrometry·Arne UlbrichJoshua J Coon
Oct 1, 1992·Journal of the American Society for Mass Spectrometry·R R Ogorzalek LooR D Smith
Jan 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·William M McGee, Scott A McLuckey
Aug 28, 2014·Analytical Chemistry·Daniel N Mortensen, Evan R Williams

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Citations

Jun 21, 2016·Rapid Communications in Mass Spectrometry : RCM·Shaan RashidPaul M Mayer
Nov 1, 2016·Journal of the American Society for Mass Spectrometry·Alice L PiloScott A McLuckey
Oct 4, 2018·Rapid Communications in Mass Spectrometry : RCM·Nan WangScott A McLuckey
Nov 6, 2018·Macromolecular Rapid Communications·Gijs RamakersTanja Junkers
May 30, 2019·Journal of the American Society for Mass Spectrometry·John R StutzmanMichael Lesniak
Jun 28, 2018·Journal of the American Society for Mass Spectrometry·Brynn N Sundberg, Anthony F Lagalante
Dec 19, 2019·Chemical Reviews·Jennifer S BrodbeltInês Santos
Nov 7, 2019·Analytical Chemistry·David Foreman, Scott A McLuckey
Sep 3, 2021·Chemical Reviews·Amber D Rolland, James S Prell

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