High-throughput biophysical measurement of human red blood cells

Lab on a Chip
Yi ZhengYu Sun

Abstract

This paper reports a microfluidic system for biophysical characterization of red blood cells (RBCs) at a speed of 100-150 cells s(-1). Electrical impedance measurement is made when single RBCs flow through a constriction channel that is marginally smaller than RBCs' diameters. The multiple parameters quantified as mechanical and electrical signatures of each RBC include transit time, impedance amplitude ratio, and impedance phase increase. Histograms, compiled from 84,073 adult RBCs (from 5 adult blood samples) and 82,253 neonatal RBCs (from 5 newborn blood samples), reveal different biophysical properties across samples and between the adult and neonatal RBC populations. In comparison with previously reported microfluidic devices for single RBC biophysical measurement, this system has a higher throughput, higher signal to noise ratio, and the capability of performing multi-parameter measurements.

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Citations

Sep 13, 2012·Lab on a Chip·Xiaole Mao, Tony Jun Huang
Apr 24, 2013·Proceedings of the National Academy of Sciences of the United States of America·Sangwon ByunScott R Manalis
Apr 29, 2014·Journal of Biomechanics·Quan GuoHongshen Ma
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Jul 12, 2014·Analytical and Bioanalytical Chemistry·Zhen ZhuAndreas Hierlemann
May 6, 2015·International Journal of Molecular Sciences·Jian ChenJunbo Wang
Jul 22, 2015·Annual Review of Biomedical Engineering·Eric M Darling, Dino Di Carlo
Jan 13, 2015·Scientific Reports·Yi ZhengYu Sun
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Jun 2, 2015·Acta Pharmacologica Sinica·Mi LiYue-chao Wang
Jan 30, 2015·Lab on a Chip·John NguyenYu Sun
Nov 21, 2012·Analytical Chemistry·Sukgyun ChaJu Min Kim
Oct 1, 2016·Micromachines·Chia-Hung Dylan TsaiYasushi Sakata

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