High-throughput cloning of Campylobacter jejuni ORfs by in vivo recombination in Escherichia coli

Journal of Proteome Research
Jodi R ParrishRussell L Finley

Abstract

A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses native E. coli recombination functions and requires no in vitro enzymatic steps or special strains. Our results indicate that this approach is an efficient and economical alternative for high-throughput cloning.

References

Oct 25, 1990·Nucleic Acids Research·C Aslanidis, P J de Jong
Nov 11, 1993·Nucleic Acids Research·J D OlinerB Vogelstein
Jul 25, 1993·Nucleic Acids Research·P BubeckW Bautsch
Nov 15, 2000·Genome Research·J L HartleyM A Brasch
Jun 20, 2002·Protein Expression and Purification·Lynda DieckmanFrank R Collart

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Citations

Oct 22, 2008·Nature Chemical Biology·Joseph LehárCorey Nislow
Dec 24, 2011·Applied and Environmental Microbiology·Charlotte Tandrup RiedelLone Brøndsted
Dec 14, 2011·Clinical and Vaccine Immunology : CVI·Lene N NielsenKaren A Krogfelt
Jul 7, 2007·Genome Biology·Jodi R ParrishRussell L Finley
Jan 27, 2010·Genetics·Dongmei Liu, Russell L Finley
Jan 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·D Huw DaviesPhilip L Felgner
Aug 13, 2011·Veterinary Microbiology·Lucas Moitinho-SilvaHenrique B Ferreira
Feb 20, 2008·Current Opinion in Chemical Biology·Yoko YashirodaMinoru Yoshida
Jun 20, 2014·Mechanisms of Development·Govindaraja AtikukkeRussell L Finley
Jun 7, 2016·Scientific Reports·Javier García-NafríaIngo H Greger
Jun 18, 2016·Analytical Biochemistry·Katrin MesserschmidtBernd Mueller-Roeber
Mar 17, 2015·PloS One·Ana Paula Jacobus, Jeferson Gross
Jul 20, 2016·Parasitology·Alemao G Carpinteyro SánchezMarilise Brittes Rott
Feb 20, 2009·Journal of the American Chemical Society·Santosh Kumar PadhiJon D Stewart
Nov 19, 2017·Scientific Reports·Kate E Royle, Karen Polizzi
Jun 8, 2018·World Journal of Microbiology & Biotechnology·Tiwa RotchanapreedaJamorn Somana
Sep 16, 2019·The Journal of Biological Chemistry·Jake F Watson, Javier García-Nafría
Nov 20, 2016·Molecular Genetics and Genomics : MGG·Taylor GonchoroskiHenrique B Ferreira
Jan 17, 2021·FEMS Microbiology Letters·Amanda Malvessi CattaniFranciele Maboni Siqueira
May 27, 2008·Journal of the American Chemical Society·Despina J Bougioukou, Jon D Stewart

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Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.