High Throughput Complementary Analysis and Quantitation of Metabolites by MALDI- and Silicon Nanopost Array-Laser Desorption/Ionization-Mass Spectrometry

Analytical Chemistry
Andrew R KorteAkos Vertes

Abstract

Silicon nanopost array (NAPA) structures have been shown to be effective substrates for laser desorption/ionization-mass spectrometry (LDI-MS) and have been used to analyze a variety of samples including peptides, metabolites, drugs, explosives, and intact cells, as well as to image lipids and metabolites in tissue sections. However, no direct comparison has yet been conducted between NAPA-MS and the most commonly used LDI-MS technique, matrix-assisted laser desorption/ionization (MALDI)-MS. In this work, we compare the utility of NAPA-MS to that of MALDI-MS using two common matrices for the analysis of metabolites in cellular extracts and human urine. Considerable complementarity of molecular coverage was observed between the two techniques. Of 178 total metabolites assigned from cellular extracts, 68 were uniquely detected by NAPA-MS and 62 were uniquely detected by MALDI-MS. NAPA-MS was found to provide enhanced coverage of low-molecular weight compounds such as amino acids, whereas MALDI afforded better detection of larger, labile compounds including nucleotides. In the case of urine, a sample largely devoid of higher-mass labile compounds, 88 compounds were uniquely detected by NAPA-MS and 13 by MALDI-MS. NAPA-MS also favo...Continue Reading

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Citations

Nov 10, 2020·Mass Spectrometry Reviews·Kellen DeLaneyLingjun Li
Mar 4, 2021·Journal of the American Society for Mass Spectrometry·Michael J TaylorChristopher R Anderton
Apr 20, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Anuja Shreeram KulkarniKun Qian
Jan 15, 2021·Annual Review of Physical Chemistry·Daisy UnsihuayJulia Laskin
Mar 16, 2021·Angewandte Chemie·Rongxin LiJingjing Wan
Jul 11, 2021·Journal of Separation Science·Wenya HuHonggang Nie
Aug 10, 2021·Journal of the American Society for Mass Spectrometry·Yaju ZhaoDanke Xu

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