High-throughput dynamical analysis of dielectrophoretic frequency dispersion of single cells based on deflected flow streamlines.

Analytical and Bioanalytical Chemistry
Karina Torres-CastroNathan S Swami

Abstract

Phenotypic quantification of cells based on their plasma membrane capacitance and cytoplasmic conductivity, as determined by their dielectrophoretic frequency dispersion, is often used as a marker for their biological function. However, due to the prevalence of phenotypic heterogeneity in many biological systems of interest, there is a need for methods capable of determining the dielectrophoretic dispersion of single cells at high throughput and without the need for sample dilution. We present a microfluidic device methodology wherein localized constrictions in the microchannel are used to enhance the field delivered by adjoining planar electrodes, so that the dielectrophoresis level and direction on flow-focused cells can be determined on each traversing cell in a high-throughput manner based on their deflected flow streamlines. Using a sample of human red blood cells diluted to 2.25 × 108 cells/mL, the dielectrophoretic translation of single cells traversing at a flow rate of 1.68 μL/min is measured at a throughput of 1.1 × 105 cells/min, to distinguish positive versus negative dielectrophoresis and determine their crossover frequency in media of differing conductivity for validation of the computed membrane capacitance to th...Continue Reading

References

Sep 1, 1971·Biophysical Journal·H A Pohl, J S Crane
Mar 4, 2000·Analytical Chemistry·X B WangP R Gascoyne
Jun 10, 2004·Electrophoresis·Blanca H Lapizco-EncinasYolanda Fintschenko
Feb 14, 2006·IEE Proceedings. Nanobiotechnology·Hywel Morgan
Feb 22, 2008·Electrophoresis·Matthew S PommerHyongsok T Soh
Mar 4, 2009·Analytical Chemistry·M D Vahey, J Voldman
Nov 19, 2009·Molecular Systems Biology·Theodore J Perkins, Peter S Swain
Mar 18, 2010·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Donald WlodkowicZbigniew Darzynkiewicz
Oct 15, 2013·Analytica Chimica Acta·Karel Klepárník, František Foret
Jan 11, 2014·Analytical and Bioanalytical Chemistry·Sanchari BhattacharyaAlexandra Ros
Mar 22, 2014·Annals of Oncology : Official Journal of the European Society for Medical Oncology·P K GroverJ E Hardingham
Mar 26, 2014·Cancers·Peter R C Gascoyne, Sangjo Shim
Apr 24, 2014·Lab on a Chip·Meltem ElitasPhilippe Renaud
Oct 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Wong Cheng LeeKrystyn J Van Vliet
Jan 16, 2016·Critical Reviews in Biotechnology·Aysun AdanAyten Nalbant
Apr 5, 2017·Analytica Chimica Acta·Renny E FernandezNathan S Swami
Dec 19, 2018·Biophysical Journal·Jonathan CottetMarie Frénéa-Robin

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Zeitschrift Für Naturforschung. C, a Journal of Biosciences
J J ZielińskiM Fikus
Journal of Theoretical Biology
H A Pohl, J S Crane
Canadian Journal of Microbiology
B D Mason, P M Townsley
© 2021 Meta ULC. All rights reserved