High throughput extraction of plant, marine and fungal specimens for preservation of biologically active molecules.

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Thomas G McCloud

Abstract

The Developmental Therapeutics Program (DTP) of the U.S. National Cancer Institute (NCI), at its NCI-Frederick facility, has built perhaps the largest and most diverse natural products screening library in the world for drug discovery. Composed of plant, marine organism and microbial extracts, it currently contains in excess of 230,000 unique materials. From the inception of this program to identify new anticancer chemotherapeutics from natural products sources in 1987, two extracts have been sequentially prepared from each specimen: one produced by organic solvent extraction, which yields a complex material that contains non- to moderately polar small molecules, and a water-soluble extract, a milieu largely unexplored for useful drugs in earlier years, which contains polar small to medium-sized molecules. Plant specimens and microbial ferments are extracted by modified traditional methods, while the method developed to produce extracts from marine organisms is unique and very different from that used by marine natural products chemists previously, but again yields both an organic solvent soluble and a water soluble material for inclusion into the screening library. Details of high throughput extract production for preservation...Continue Reading

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Citations

Nov 12, 2010·Journal of Natural Products·Emily L WhitsonTawnya C McKee
Apr 15, 2011·Journal of Natural Products·Albert W W Van WykTawnya C McKee
Feb 11, 2011·Journal of Natural Products·Krishna P DevkotaJohn A Beutler
Feb 1, 2011·Journal of Natural Products·Muhammad Ali VersianiKirk R Gustafson
Aug 3, 2011·Journal of Natural Products·Wendy L PopplewellTawnya C McKee
Mar 1, 2012·Journal of Natural Products·Jonathan SorresAli Al-Mourabit
Feb 9, 2012·Journal of Natural Products·Emily L WhitsonTawnya C McKee
Jan 28, 2012·Journal of Natural Products·Rhone K AkeeJohn A Beutler
Aug 30, 2012·Journal of Natural Products·Tawnya C McKeeDonald P Bottaro
Jul 28, 2012·Journal of Natural Products·Thushara DiyabalanageKirk R Gustafson
Feb 8, 2013·Journal of Natural Products·Manjeet KumarBhahwal Ali Shah
Mar 23, 2013·Journal of Natural Products·Krishna P DevkotaJohn A Beutler
Jun 5, 2013·Journal of Natural Products·Lin DuDale G Nagle
Oct 29, 2013·BMC Complementary and Alternative Medicine·Rahsan Ilikci Sagkan
Sep 2, 2011·Chemical & Pharmaceutical Bulletin·Heidi Rose BokeschTawnya Carlene McKee
Jan 9, 2014·British Poultry Science·M BozkurtP Florou-Paneri
Jan 21, 2014·Phytochemistry Letters·Krishna P DevkotaJohn A Beutler
Jul 8, 2014·Journal of Medicinal Food·Zheng TuT Alan Jiang
Aug 9, 2013·Natural Product Reports·Curtis J Henrich, John A Beutler
Jan 19, 2016·Phytochemical Analysis : PCA·Amber Gunderwala, John Porter
Jul 8, 2011·Bioorganic & Medicinal Chemistry Letters·Thushara DiyabalanageKirk R Gustafson
Mar 1, 2011·Bioorganic & Medicinal Chemistry Letters·Tanja GrkovicTawnya C McKee
Oct 23, 2014·Journal of Natural Products·Suthananda N SunasseeKirk R Gustafson
Feb 1, 2015·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Okiemute Rosa Johnson-AjinwoWen-Wu Li
Dec 24, 2011·Natural Product Reports·John W BluntMichèle R Prinsep
May 3, 2016·Journal of Pharmaceutical and Biomedical Analysis·Tyler N GrafNicholas H Oberlies
Apr 27, 2016·Trends in Biotechnology·Aleksandra SkiryczGeorge Tzotzos
Jan 1, 2013·Antioxidants·Mona A Abou ElfotohMahmoud A Saleh
Nov 28, 2018·Chemistry & Biodiversity·Tijana ĐorđevićJelena Gajić Umiljendić
Feb 6, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Francisco Cen-PachecoJosé Javier Fernández
Aug 6, 2019·Magnetic Resonance in Chemistry : MRC·Trong D TranKirk R Gustafson

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Methods Mentioned

BETA
column chromatography

Software Mentioned

NEVER
dBase

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