High-throughput fluorescence-activated nanoscale subcellular sorter with single-molecule sensitivity.

The Journal of Physical Chemistry. B
Perry G SchiroDaniel T Chiu

Abstract

Recent single-cell and single-molecule studies have shown that a variety of subpopulations exist within biological systems, such as synaptic vesicles, that have previously been overlooked in common bulk studies. By isolating and enriching these various subpopulations, detailed analysis with a variety of analytical techniques can be done to further understand the role that various subpopulations play in cellular dynamics and how alterations to these subpopulations affect the overall function of the biological system. Previous sorters lack the sensitivity, sorting speed, and efficiency to isolate synaptic vesicles and other nanoscale systems. This paper describes the development of a fluorescence-activated nanoscale subcellular sorter that can sort nearly 10 million objects per hour with single-molecule sensitivity. Utilizing a near-nanoscale channel system, we were able to achieve upward of 91% recovery of desired objects with a 99.7% purity.

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Citations

Jan 11, 2014·Journal of the American Chemical Society·Jing GuAndrew B Bocarsly
Jun 15, 2012·Current Opinion in Chemical Biology·Robbyn K Anand, Daniel T Chiu
Jan 28, 2017·Lab on a Chip·Remo FriedrichMarta Bally
Mar 6, 2019·The Review of Scientific Instruments·Abhay KotnalaWei Cheng
Dec 22, 2020·Small·Michael J SiedlikDavid Issadore
Mar 17, 2021·Wellcome Open Research·Alexander G BuryGavin Hudson
Mar 31, 2021·Analytical Chemistry·Luca A AndronicoDaniel T Chiu

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