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High-throughput functional genomics using CRISPR-Cas9

Nature Reviews. Genetics

Apr 10, 2015

Ophir ShalemFeng Zhang

Abstract

Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unb...read more

Mentioned in this Paper

Genome
CRISPR-Cas Systems
Cosuppression
Genetic Screening Method
Transcription, Genetic
Genetic Screening (Procedure)
Genomic Screening
Genomics
Nuclease
High Throughput Analysis
79
1
11
2357
Paper Details
References
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  • Citations219
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  • References124
  • Citations219
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High-throughput functional genomics using CRISPR-Cas9

Nature Reviews. Genetics

Apr 10, 2015

Ophir ShalemFeng Zhang

PMID: 25854182

DOI: 10.1038/nrg3899

Abstract

Forward genetic screens are powerful tools for the discovery and functional annotation of genetic elements. Recently, the RNA-guided CRISPR (clustered regularly interspaced short palindromic repeat)-associated Cas9 nuclease has been combined with genome-scale guide RNA libraries for unb...read more

Mentioned in this Paper

Genome
CRISPR-Cas Systems
Cosuppression
Genetic Screening Method
Transcription, Genetic
Genetic Screening (Procedure)
Genomic Screening
Genomics
Nuclease
High Throughput Analysis
79
1
11
2357

Similar Papers Found In These Feeds

CRISPR (general)

Clustered regularly interspaced short palindromic repeats (CRISPR) are DNA sequences in the genome that are recognized and cleaved by CRISPR-associated proteins (Cas). CRISPR-Cas system enables the editing of genes to create or correct mutations. Discover the latest research on CRISPR here.

RNAi & Gene Silencing

RNA Interference (RNAi) pathway is involved in post-transcriptional gene silencing, transcriptional silencing and epigenetic silencing as well as its use as a tool for forward genetics and therapeutics. Discover the latest research here.

Related Papers

Frontiers in Genetics

A CRISPR CASe for high-throughput silencing

Frontiers in GeneticsOctober 11, 2013
Jacob HeintzeRobin Ketteler
Methods in Enzymology

Adapting CRISPR/Cas9 for functional genomics screens

Methods in EnzymologyNovember 16, 2014
Abba MalinaJerry Pelletier
Paper Details
References
  • References124
  • Citations219
12345...
  • References124
  • Citations219
12345...
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