High-throughput identification of the sensitivities of an Escherichia coli ΔrecA mutant strain to various chemical compounds

The Journal of Antibiotics
Tomoya MaedaChikara Furusawa

Abstract

Antibiotic resistance is considered a global threat to public health. Adaptive resistance mutations and the acquisition of resistance genes by horizontal gene transfer are known to be facilitated by the RecA-dependent SOS response during antibiotic treatment, making RecA inhibitors promising agents for the prevention of antibiotic resistance. However, the impact of RecA inactivation on antibiotic sensitivities remains unclear. Therefore, in this study, we performed high-throughput screening to determine the minimum inhibitory concentrations (MICs) of 217 chemicals, including both antibiotics and toxic chemicals of unknown drug action, in the wild-type MDS42 and the ΔrecA mutant strains of Escherichia coli. The ΔrecA mutant showed increased sensitivity to DNA-damaging agents, DNA replication inhibitors, and chromate stress, as well as to other chemicals, such as S-(2-aminoethyl)-L-cysteine, L-histidine, ruthenium red, D-penicillamine, carbonyl cyanide 3-chlorophenylhydrazone (CCCP), cerulenin, and L-cysteine. Microarray analysis showed further that the ΔrecA mutant had lower expressions of glnK, nac, and glnLG, which encode nitrogen assimilation regulators, as well as amtB, which encodes an ammonium transporter, compared with th...Continue Reading

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Citations

May 13, 2020·Biophysics Reviews·Takaaki Horinouchi, Chikara Furusawa
Nov 26, 2020·Nature Communications·Tomoya MaedaChikara Furusawa
Sep 16, 2021·Microbiology and Molecular Biology Reviews : MMBR·Kam Pou Ha, Andrew M Edwards

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Datasets Mentioned

BETA
GSE121218

Methods Mentioned

BETA
PCR
Feature Extraction

Software Mentioned

Feature Extraction

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