Abstract
Wavelength interrogation surface plasmon resonance imaging (λSPRi) has potential in detecting 2-dimensional (2D) sensor array sites, but the resonance wavelength imaging rate limits the application of detecting biomolecular binding process in real time. In this paper, we have successfully demonstrated an ultrafast λSPRi biosensor system. The key feature is a two-point tracking algorithm that drives the liquid crystal tunable filter (LCTF) to achieve fast-tracking of the resonance wavelength movement caused by the binding of target molecules with the probe molecules on the sensing surface. The resonance wavelength measurement time is within 0.25s. To date, this is the fastest speed ever reported in λSPRi. Experiment results show that the sensitivity and dynamic are 2.4 × 10-6 RIU and 4.6 × 10-2 RIU, respectively. In addition, we have also demonstrated that the system has the capability of performing fast high-throughput detection of biomolecular interactions, which confirms that this fast real-time detecting approach is most suitable for high-throughput and label-free biosensing applications.
References
Mar 12, 2004·Annual Review of Physical Chemistry·W Knoll
Sep 21, 2004·Proteomics·Jong Seol YukKwon-Soo Ha
Jul 13, 2005·Applied Optics·Soichi OtsukiS Wakida
Aug 13, 2005·Biosensors & Bioelectronics·Jong Seol YukKwon-Soo Ha
Aug 25, 2007·Biosensors & Bioelectronics·X D HoaM Tabrizian
Oct 22, 2008·Applied Optics·Le LiuJihua Guo
Dec 17, 2008·Biosensors & Bioelectronics·Fabrice BardinMichael Canva
Sep 22, 2009·Biosensors & Bioelectronics·Simona ScaranoMaria Minunni
Sep 23, 2009·Optics Express·Marek Piliarik, Jirí Homola
Mar 4, 2011·Optics Express·Siu Pang NgHo Pui Ho
Nov 28, 2013·Biosensors & Bioelectronics·A SeredaE Maillart
Dec 10, 2016·Journal of Biomedical Optics·Kaiqiang ChenYonghong Shao
Dec 14, 2016·Optics Express·Youjun ZengYonghong Shao
Jan 10, 2017·Sensors·Youjun ZengYonghong Shao
Sep 29, 2019·Biosensors & Bioelectronics·Youjun ZengYonghong Shao