High-Throughput Immunochemical Method to Assess the 2-Heptyl-4-quinolone Quorum Sensing Molecule as a Potential Biomarker of Pseudomonas aeruginosa Infections.

ACS Infectious Diseases
Enrique J MontagutM Pilar Marco

Abstract

Bacterial quorum sensing (QS) is being contemplated as a promising target for developing innovative diagnostic and therapeutic strategies. Here we report for the first time the development of antibodies against 2-heptyl-4-quinolone (HHQ), a signaling molecule from the pqs QS system of Pseudomonas aeruginosa, involved in the production of important virulent factors and biofilm formation. The antibodies produced were used to develop an immunochemical diagnostic approach to assess the potential of this molecule as a biomarker of P. aeruginosa infection. The ELISA developed is able to reach a detectability in the low nM range (IC50 = 4.59 ± 0.29 nM and LOD = 0.34 ± 0.13 nM), even in complex biological samples such as Müeller Hinton (MH) culture media. The ELISA developed is robust and reproducible and has been found to be specific to HHQ, with little interference from other related alkylquinolones from the pqs QS system. The ELISA has been used to analyze the HHQ production kinetics of P. aeruginosa clinical isolates grown in MH media, pointing to its potential as a biomarker of infection and at the possibility to use the technology developed to obtain additional information about the disease stage.

References

Nov 12, 2002·Journal of Bacteriology·David A D'ArgenioEverett C Pesci
Jan 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric DézielLaurence G Rahme
Oct 7, 2004·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Tim W R LeeJames M Littlewood
Nov 30, 2004·Trends in Molecular Medicine·Gee W LauFansheng Kong
May 1, 2007·Pediatric Pulmonology·J M CourtneyJ S Elborn
Jul 24, 2007·The Journal of Pediatrics·Michael W KonstanUNKNOWN Scientific Advisory Group and the Investigators and Coordinators of the Epidemiologic Study of Cystic Fibrosis
Oct 10, 2007·Environmental Microbiology·Matthew P FletcherPaul Williams
Oct 1, 2010·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F Bittar, J-M Rolain
Jun 29, 2012·Applied Microbiology and Biotechnology·Christine Müller, Susanne Fetzner
Oct 12, 2012·Organic & Biomolecular Chemistry·F Jerry ReenGerard P McGlacken
Oct 12, 2012·PloS One·Theerthankar Das, Mike Manefield
Oct 8, 2013·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Jane L Burns, Jean-Marc Rolain
Aug 19, 2014·Analytical Chemistry·Eric J LanniJonathan V Sweedler
Sep 6, 2014·Current Pharmaceutical Design·Gilles Brackman, Tom Coenye
Sep 25, 2014·Protein & Cell·Jasmine Lee, Lianhui Zhang
Oct 4, 2014·Clinical Chemistry·Robin Patel
Dec 2, 2015·The ISME Journal·Robert A QuinnForest L Rohwer
Jan 26, 2016·Journal of Medicinal Chemistry·Stefanie WagnerAlexander Titz
Aug 12, 2016·Nature Reviews. Microbiology·Kai Papenfort, Bonnie L Bassler
Oct 16, 2016·American Journal of Infection Control·Víctor Daniel RosenthalUNKNOWN Remaining authors
Oct 25, 2016·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Helen L BarrAndrew W Fogarty
Apr 20, 2017·Frontiers in Cellular and Infection Microbiology·An HotterbeekxSurbhi Malhotra-Kumar
Nov 23, 2017·Current Opinion in Microbiology·Martina ValentiniAlain Filloux
May 18, 2018·ACS Infectious Diseases·Lori L Burrows
Mar 12, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Hui ChenPing Wang
Apr 10, 2019·American Journal of Infection Control·Jean-Baptiste DenisLaurent Papazian
Sep 19, 2019·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·G TaccettiI Sermet-Gaudelus
Oct 30, 2019·Analytical Chemistry·Eike Ulrich BrockmannKlaus Dreisewerd

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