High-throughput in planta expression screening identifies an ADP-ribosylation factor (ARF1) involved in non-host resistance and R gene-mediated resistance.

Molecular Plant Pathology
Bert CoemansRyohei Terauchi

Abstract

To identify positive regulators of cell death in plants, we performed a high-throughput screening, employing potato virus X-based overexpression in planta of a cDNA library derived from paraquat-treated Nicotiana benthamiana leaves. The screening of 30,000 cDNA clones enabled the identification of an ADP-ribosylation factor 1 (ARF1) that induces cell death when overexpressed in N. benthamiana. Overexpression of the guanosine diphosphate (GDP)-locked mutant of ARF1 did not trigger cell death, suggesting that ARF1 guanosine triphosphatase (GTPase) activity is necessary for the observed cell death-inducing activity. The ARF1 transcript level increased strongly following treatment with Phytophthora infestans elicitor INF1, as well as inoculation with a non-host pathogen Pseudomonas cichorii in N. benthamiana. In addition, ARF1 was induced in the interaction between the N gene and tobacco mosaic virus (TMV) in Nicotiana tabacum. By contrast, inoculation with the virulent pathogen Pseudomonas syringae pv. tabaci did not affect ARF1 expression in N. benthamiana. Virus-induced gene silencing of ARF1 in N. benthamiana resulted in a stunted phenotype, and severely hampered non-host resistance towards P. cichorii. In addition, ARF1 silenc...Continue Reading

References

May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R A KahnG M Bokoch
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L MonacoM Vaughan
Jan 6, 1995·The Journal of Biological Chemistry·R A KahnM Cavenagh
Jan 28, 1994·Science·S CockcroftJ J Hsuan
Feb 1, 1994·The Journal of Cell Biology·C J ZhangR A Kahn
Mar 1, 1997·The Plant Journal : for Cell and Molecular Biology·T Aoyama, N H Chua
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Apr 4, 1998·Plant Molecular Biology·E E KarrerC A Holt
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·T KawasakiK Shimamoto
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·F L TakkenM H Joosten
Jan 10, 2001·Proceedings of the National Academy of Sciences of the United States of America·E OnoK Shimamoto
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·A Kobayashi-UeharaH Handa
Feb 13, 2001·The Plant Journal : for Cell and Molecular Biology·F RatcliffD C Baulcombe
Feb 24, 2001·Plant Molecular Biology·M C Heath
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·T Kirchhausen
Aug 2, 2001·Biochemical and Biophysical Research Communications·O H Shin, J H Exton
Dec 26, 2001·Science's STKE : Signal Transduction Knowledge Environment·G G Simpson, C Dean
May 15, 2002·Molecular Biology of the Cell·Ayanthi A RichardsRobert G Parton
Sep 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Utut SuharsonoKo Shimamoto
Nov 2, 2002·The Plant Journal : for Cell and Molecular Biology·Dinah QutobMark Gijzen
Jul 2, 2003·FEBS Letters·Ganesh K AgrawalRandeep Rakwal
Aug 2, 2003·Current Opinion in Cell Biology·Zhongzhen NiePaul A Randazzo
Apr 23, 2004·Trends in Plant Science·Kirankumar S Mysore, Choong-Min Ryu
May 6, 2004·The Plant Journal : for Cell and Molecular Biology·Inmaculada ContrerasFernando Aniento
Jul 9, 2004·Biochimica Et Biophysica Acta·Abdul R Memon
Sep 1, 2004·Current Opinion in Plant Biology·Ying GuZhenbiao Yang
Oct 20, 2004·Current Opinion in Plant Biology·Arthur J MolendijkKlaus Palme

❮ Previous
Next ❯

Citations

Dec 9, 2008·Molecular Plant-microbe Interactions : MPMI·Yoshihiro TakahashiRyohei Terauchi
Oct 26, 2012·Journal of Virology·Kiwamu HyodoTetsuro Okuno
Aug 4, 2009·The New Phytologist·Rients E Niks, Thierry C Marcel
Sep 9, 2015·Journal of Integrative Plant Biology·Wen-Ming WangShun-Yuan Xiao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.