High-throughput in vivo vertebrate screening.

Nature Methods
Carlos Pardo-MartinMehmet Fatih Yanik

Abstract

We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.

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Citations

Dec 7, 2013·Molecules and Cells·Morgan Hamon, Jong Wook Hong
Feb 22, 2013·Chemical Reviews·Venkataragavalu Sivagnanam, Martin A M Gijs
Feb 14, 2013·Nature Communications·Carlos Pardo-MartinMehmet Fatih Yanik
Dec 31, 2010·Nature Methods·Monya Baker
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Jul 16, 2011·Annual Review of Biomedical Engineering·Mehmet Fatih YanikCarlos Pardo-Martin
Sep 15, 2012·Annual Review of Genetics·David Staudt, Didier Stainier
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Methods Mentioned

BETA
fluorescence
confocal microscopy
fluorescence imaging

Software Mentioned

VAST

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