PMID: 20639868DOI: 10.1038/nmeth.1481Jul 20, 2010Paper
High-throughput in vivo vertebrate screening.
Nature Methods
Carlos Pardo-MartinMehmet Fatih Yanik
Abstract
We demonstrate a high-throughput platform for cellular-resolution in vivo chemical and genetic screens on zebrafish larvae. The system automatically loads zebrafish from reservoirs or multiwell plates, and positions and rotates them for high-speed confocal imaging and laser manipulation of both superficial and deep organs within 19 s without damage. We performed small-scale test screening of retinal axon guidance mutants and neuronal regeneration assays in combination with femtosecond laser microsurgery.
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Methods Mentioned
BETA
fluorescence
confocal microscopy
fluorescence imaging
Software Mentioned
VAST
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