High-throughput intensity diffraction tomography with a computational microscope

Biomedical Optics Express
Ruilong LingLei Tian

Abstract

We demonstrate a motion-free intensity diffraction tomography technique that enables the direct inversion of 3D phase and absorption from intensity-only measurements for weakly scattering samples. We derive a novel linear forward model featuring slice-wise phase and absorption transfer functions using angled illumination. This new framework facilitates flexible and efficient data acquisition, enabling arbitrary sampling of the illumination angles. The reconstruction algorithm performs 3D synthetic aperture using a robust computation and memory efficient slice-wise deconvolution to achieve resolution up to the incoherent limit. We demonstrate our technique with thick biological samples having both sparse 3D structures and dense cell clusters. We further investigate the limitation of our technique when imaging strongly scattering samples. Imaging performance and the influence of multiple scattering is evaluated using a 3D sample consisting of stacked phase and absorption resolution targets. This computational microscopy system is directly built on a standard commercial microscope with a simple LED array source add-on, and promises broad applications by leveraging the ubiquitous microscopy platforms with minimal hardware modificat...Continue Reading

References

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Jul 30, 2014·Biomedical Optics Express·Lei TianLaura Waller
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May 15, 2015·Optics Express·Lei Tian, Laura Waller
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Nov 22, 2016·Biomedical Optics Express·Michael ChenLaura Waller
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Jan 4, 2018·Biomedical Optics Express·José A RodrigoTatiana Alieva

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Citations

Mar 25, 2020·Biomedical Optics Express·Alex MatlockLei Tian
Apr 1, 2020·Optics Express·Pavan Chandra KondaRoarke Horstmeyer
Dec 25, 2019·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·Arnold D Kim, Chrysoula Tsogka
Aug 31, 2019·Biomedical Optics Express·Li-Hao YehLaura Waller
May 15, 2020·Optics Express·Kevin C Zhou, Roarke Horstmeyer
Sep 11, 2020·Optics Express·Zhenxiang LuoLiesbet Lagae

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