High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M MortimerAnne Kahru

Abstract

Despite of the growing production and use of nanoparticles (NPs) in various applications, current regulations, including EC new chemical policy REACH, fail to address the environmental, health, and safety risks posed by NPs. This paper shows that kinetic Vibrio fischeri luminescence inhibition test--Flash Assay--that up to now was mainly used for toxicity analysis of solid and colored environmental samples (e.g. sediments, soil suspensions), is a powerful tool for screening the toxic properties of NPs. To demonstrate that Flash Assay (initially designed for a tube luminometer) can also be adapted to a microplate format for high throughput toxicity screening of NPs, altogether 11 chemicals were comparatively analyzed. The studied chemicals included bulk and nanosized CuO and ZnO, polyethylenimine (PEI) and polyamidoamine dendrimer generations 2 and 5 (PAMAM G2 and G5). The results showed that EC50 values of 30-min Flash Assay in tube and microplate formats were practically similar and correlated very well (log-logR2=0.98), classifying all analyzed chemicals, except nano CuO (that was more toxic in cuvette format), analogously when compared to the risk phrases of the EC Directive 93/67/EEC for ranking toxicity of chemicals for aq...Continue Reading

References

Nov 14, 1997·Microbiology·Ilkka M HelanderPertti Koski
Jul 30, 1999·Journal of Controlled Release : Official Journal of the Controlled Release Society·W T GodbeyA G Mikos
Nov 25, 2005·Advanced Drug Delivery Reviews·Sönke Svenson, Donald A Tomalia
Feb 4, 2006·Science·Andre NelNing Li
Apr 25, 2007·Critical Reviews in Toxicology·Gerhard J NohynekMichael S Roberts
Apr 11, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M MortimerA Kahru
Feb 1, 2006·Environmental Toxicology and Pharmacology·Sandra CoeckeThomas Hartung

❮ Previous
Next ❯

Citations

Feb 11, 2014·Ecotoxicology and Environmental Safety·Carlo PrettiCinzia Chiappe
Jan 5, 2014·Journal of Hazardous Materials·Javier Santiago-MoralesAmadeo R Fernández-Alba
May 20, 2014·Ecotoxicology and Environmental Safety·Xin YuFei Zhang
May 29, 2014·Environmental Science and Pollution Research International·Liina KanarbikAnne Kahru
Dec 18, 2013·Environmental Science and Pollution Research International·Rebeca ManzanoPaula Alvarenga
Oct 8, 2011·International Journal of Nanomedicine·Xin WuShen Gao
Nov 7, 2008·Analytical and Bioanalytical Chemistry·Marinella FarréDamià Barceló
Nov 11, 2009·Analytical and Bioanalytical Chemistry·Stella W Y WongKenneth M Y Leung
Nov 26, 2013·Nanotoxicology·Eva OliveiraBenjamin Piña
Feb 25, 2011·Chemosphere·Shawna NationsGeorge P Cobb
Aug 31, 2010·Ecotoxicology and Environmental Safety·Shawna NationsGeorge P Cobb
Mar 9, 2010·Aquatic Toxicology·Anne-Noëlle PetitFrançois Gagné
Jun 3, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Kaja KasemetsAnne Kahru
Jan 23, 2014·Environmental Pollution·Nicole MartineauAnne J Anderson
Jan 22, 2013·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Bengt FadeelWolfgang J Parak
Jul 21, 2015·Chemical Society Reviews·Kristen P MillerAlan W Decho
Feb 15, 2012·Environmental Toxicology·François PerreaultWilliam Gerson Matias
Jul 16, 2015·Ecotoxicology·Shona O'RourkeTeresa F Fernandes
Apr 24, 2015·Environmental Science and Pollution Research International·Daniel S ReadClaus Svendsen
Jun 8, 2014·The Science of the Total Environment·Ana Letícia de O F RossettoWilliam Gerson Matias
Mar 26, 2014·The Science of the Total Environment·Fabian P SteinmetzMark T D Cronin
Jun 12, 2016·Toxicology and Applied Pharmacology·Lambert BodeweinMartina Fenske
Apr 11, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·M MortimerA Kahru
Nov 15, 2012·Accounts of Chemical Research·Anne Kahru, Angela Ivask
Nov 28, 2012·The Journal of Physical Chemistry. C, Nanomaterials and Interfaces·Priyanka BhattacharyaPu Chun Ke
Sep 25, 2016·Environmental Science and Pollution Research International·Natalia SzczepańskaBłażej Kudłak
Apr 26, 2017·Environmental Toxicology and Chemistry·Andrew BarrickCatherine Mouneyrac
Feb 15, 2018·International Journal of Environmental Research and Public Health·Pratap C NahaHugh J Byrne
Nov 10, 2017·Frontiers in Microbiology·Alice RotiniLuciana Migliore
Nov 23, 2015·Archives of Environmental Contamination and Toxicology·Irina BlinovaAnne Kahru
Sep 6, 2014·Environmental Science and Pollution Research International·Marta WagilStefan Stolte
Feb 13, 2013·Physical Chemistry Chemical Physics : PCCP·Priyanka BhattacharyaPu Chun Ke

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.