High-throughput loss-of-heterozygosity study of chromosome 3p in lung cancer using single-nucleotide polymorphism markers

Cancer Research
Amy L S TaiXin-Yuan Guan

Abstract

Loss of DNA copy number at the short arm of chromosome 3 is one of the most common genetic changes in human lung cancer, suggesting the existence of one or more tumor suppressor genes (TSG) at 3p. To identify most frequently deleted regions and candidate TSGs within these regions, a recently developed single-nucleotide polymorphism (SNP)-mass spectrometry-genotyping (SMSG) technology was applied to investigate the loss of heterozygosity (LOH) in 30 primary non-small-cell lung cancers. A total of 386 SNP markers that spanned a region of 70 Mb at 3p, from 3pter to 3p14.1, were selected for LOH analysis. The average intermarker distance in the present study is approximately 180 kb. Several frequently deleted regions, including 3p26.3, 3p25.3, 3p24.1, 3p23, and 3p21.1, were found. Several candidate TSGs within these frequently detected LOH regions have been found, including APG7L at 3p25.3, CLASP2 at 3p23, and CACNA2D3 at 3p21.1. This study also showed that SMSG technology is a very useful approach to rapidly define the minimal deleted region and to identify target TSGs in a given cancer.

References

Oct 29, 1992·Nature·J WeissenbachM Lathrop
Jan 1, 1996·CA: a Cancer Journal for Clinicians·S L ParkerP A Wingo
Apr 5, 1996·Cell·G SozziC M Croce
Oct 6, 1998·Nature·N MizushimaY Ohsumi
Feb 22, 2001·Journal of the National Cancer Institute·A JemalR E Tarone
Mar 10, 2001·Nature·R SachidanandamUNKNOWN International SNP Map Working Group
Mar 26, 2003·Nature Genetics·David E ReichDavid Altshuler
Mar 28, 2003·Genomics·Catherine I DumurAndrea Ferreira-Gonzalez
Sep 10, 2003·Nature Biotechnology·Giulia C KennedyKeith W Jones

❮ Previous
Next ❯

Citations

Jul 14, 2011·Proceedings of the National Academy of Sciences of the United States of America·Akira WatanabeHiroyuki Aburatani
Oct 27, 2007·American Journal of Respiratory and Critical Care Medicine·Giovanna E CarpagnanoAngelo Paradiso
Jul 28, 2009·Journal of Experimental & Clinical Cancer Research : CR·Tadahiko NakamuraHideo Baba
Mar 1, 2012·Chinese Journal of Cancer·Juan ChenXin-Yuan Guan
Oct 6, 2014·The Annals of Thoracic Surgery·Koei IkedaMakoto Suzuki
Dec 7, 2007·The Bulletin of Tokyo Dental College·Nobuharu YamamotoChitta Choudhury
Jul 9, 2008·Acta Oncologica·Zhang CaoWon Sang Park
May 21, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Christina I SelingerMaija R J Kohonen-Corish
Feb 5, 2008·International Journal of Cancer. Journal International Du Cancer·Jing ChenJian-Bing Fan
Dec 20, 2007·International Journal of Cancer. Journal International Du Cancer·Yanlei GuanKenshi Hayashi
May 8, 2013·International Journal of Cancer. Journal International Du Cancer·Alissa Michelle Go WongXin-Yuan Guan
Feb 24, 2015·Biochimica Et Biophysica Acta·Halima Ouadid-AhidouchAhmed Ahidouch
Sep 10, 2013·Seminars in Ophthalmology·Xiang Q WerdichIvana K Kim
Sep 2, 2016·Molecular Carcinogenesis·Yi JinLiang Gao
Feb 1, 2011·Virchows Archiv : an International Journal of Pathology·Xiao-Jie DingYi Cao
Jun 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael D OnkenJ William Harbour
Dec 9, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ewa DudziecJames W F Catto
May 17, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Michael D OnkenJ William Harbour

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.