High-throughput measurement of 25-hydroxyvitamin D by LC-MS/MS with separation of the C3-epimer interference for pediatric populations

Clinica Chimica Acta; International Journal of Clinical Chemistry
Yifei YangJoe M El-Khoury

Abstract

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is widely used for the measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in blood. However, the presence of 25OHD3 C3-epimer (3-epi-25OHD3) may cause interference and overestimation of the 25OHD3 level. We developed a rapid and simple assay for measurement of 25OHD that separates this interference and to investigate its impact on 25OHD3 measurement in adult and pediatric populations. Sample preparation consisted of protein precipitation followed by solid-phase extraction with an LC run time of 4.8 min. Method comparison with another LC-MS/MS method of a major reference laboratory that does not separate the C3-epimer interference was performed using adult (n=52) and pediatric (n=40) samples. This method is free from significant ion suppression, carryover and interference. The assay can separate 25OHD3 from the 3-epi-25OHD3, and can measure 25OHD2 and 25OHD3 from 4.2 to 310.7 ng/ml and 5.2 to 311.1 ng/ml, respectively. Method comparison with a LC-MS method that does not separate the interference revealed biases of -0.15 and 4.54 for 25OHD3 measurement in adult and pediatric samples, respectively. A fast and simple LC-MS/MS method for quantifica...Continue Reading

References

Mar 30, 2011·Journal of the American Dietetic Association·A Catharine RossSue A Shapses
Oct 11, 2011·Clinica Chimica Acta; International Journal of Clinical Chemistry·Frederick G StrathmannRhona Jack
Aug 28, 2013·Clinica Chimica Acta; International Journal of Clinical Chemistry·Mary BednerSusan S-C Tai
Mar 31, 2015·Diabetes & Metabolic Syndrome·Priyanka Prasad, Anita Kochhar
Apr 22, 2015·Integrated Blood Pressure Control·Liang KeKaye E Brock

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Citations

Jan 17, 2020·International Journal of Molecular Sciences·Bashar Al-ZohilyIltaf Shah
Mar 8, 2020·International Journal of Environmental Research and Public Health·Marta PelczyńskaKrystyna Czyżewska
Nov 22, 2020·Reproductive Biology and Endocrinology : RB&E·Samantha SimpsonAlla Vash-Margita
Mar 14, 2021·Clinica Chimica Acta; International Journal of Clinical Chemistry·Michael J BinksJacobus Ungerer
Nov 29, 2021·Current Opinion in Endocrinology, Diabetes, and Obesity·Ashley J StoffersMichael A Levine

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