High-Throughput Metabolomics by 1D NMR

Angewandte Chemie
Alessia VignoliClaudio Luchinat

Abstract

Metabolomics deals with the whole ensemble of metabolites (the metabolome). As one of the -omic sciences, it relates to biology, physiology, pathology and medicine; but metabolites are chemical entities, small organic molecules or inorganic ions. Therefore, their proper identification and quantitation in complex biological matrices requires a solid chemical ground. With respect to for example, DNA, metabolites are much more prone to oxidation or enzymatic degradation: we can reconstruct large parts of a mammoth's genome from a small specimen, but we are unable to do the same with its metabolome, which was probably largely degraded a few hours after the animal's death. Thus, we need standard operating procedures, good chemical skills in sample preparation for storage and subsequent analysis, accurate analytical procedures, a broad knowledge of chemometrics and advanced statistical tools, and a good knowledge of at least one of the two metabolomic techniques, MS or NMR. All these skills are traditionally cultivated by chemists. Here we focus on metabolomics from the chemical standpoint and restrict ourselves to NMR. From the analytical point of view, NMR has pros and cons but does provide a peculiar holistic perspective that may ...Continue Reading

References

Sep 20, 1993·Annals of the New York Academy of Sciences·M Navazesh
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Aug 18, 2000·Neural Networks : the Official Journal of the International Neural Network Society·A Hyvärinen, E Oja
Apr 5, 2003·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Magnus Astrand
Aug 25, 2004·Current Biology : CB·Fred Kippert, Dietlind L Gerloff
Feb 24, 2006·Journal of the American Chemical Society·Gerhard Wider, Lars Dreier
Apr 21, 2006·Nature·T Andrew ClaytonJeremy K Nicholson
Oct 7, 2006·Journal of Proteome Research·Cinzia StellaJeremy K Nicholson
Jan 5, 2007·Nucleic Acids Research·David S WishartLori Querengesser
May 16, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Wederson Marcos ClaudinoAngelo Di Leo
Jan 31, 2008·Proceedings of the National Academy of Sciences of the United States of America·Michael AssfalgManfred Spraul
Feb 7, 2008·Physiologia Plantarum·Robert D HallMelissa A Fitzgerald
Mar 11, 2008·Nature Biotechnology·Markus Ringnér
May 27, 2008·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·David S WishartLiang Li
Jul 26, 2008·The European Respiratory Journal·G de LaurentiisM Sofia
Aug 16, 2008·Analytical Chemistry·Jake T M PearceHector C Keun
Oct 25, 2008·Nature·Jeremy K Nicholson, John C Lindon
Oct 28, 2008·Nucleic Acids Research·David S WishartIan Forsythe
Dec 17, 2008·Journal of Proteome Research·Ivano BertiniLeonardo Tenori
Jan 17, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jennifer L SpratlinS Gail Eckhardt
Feb 19, 2009·Journal of Proteome Research·Ann L Oberg, Olga Vitek
Jun 17, 2009·Journal of Proteome Research·Patrizia BerniniLeonardo Tenori
Sep 5, 2009·Magnetic Resonance in Chemistry : MRC·Miroslava Cuperlović-CulfJohn A Walter
Sep 22, 2009·Journal of Chromatography. a·Alejandro Cifuentes
Nov 10, 2009·Magnetic Resonance in Chemistry : MRC·Manfred SpraulPeter Rinke
Dec 17, 2009·Journal of Magnetic Resonance·F SavoraniS B Engelsen
Jan 5, 2010·Metabolomics : Official Journal of the Metabolomic Society·Augustin ScalbertSuzan Wopereis
Feb 18, 2010·Biomarkers in Medicine·Andrea L Merz, Natalie J Serkova
May 13, 2010·The International Journal of Biochemistry & Cell Biology·Catherine OakmanAngelo Di Leo
Jun 12, 2010·Nature Protocols·Olaf BeckonertJeremy K Nicholson
Jul 19, 2008·Annual Review of Analytical Chemistry·John C Lindon, Jeremy K Nicholson
Oct 21, 2010·Cancer Research·Vincent M AsiagoDaniel Raftery
Nov 10, 2010·Journal of Proteome Research·Joana CarrolaIola F Duarte
Nov 26, 2010·Journal of Proteome Research·Patrizia BerniniLeonardo Tenori
Feb 19, 2011·Omics : a Journal of Integrative Biology·Andrew N LaneDonald M Miller
Mar 2, 2011·PloS One·Nikolaos PsychogiosDavid S Wishart

❮ Previous
Next ❯

Citations

Aug 10, 2019·Angewandte Chemie·Frans A A MulderClaudio Luchinat
Nov 16, 2019·Angewandte Chemie·Islam AlshamlehHarald Schwalbe
Feb 7, 2020·Expert Opinion on Drug Discovery·Kevin Andrew StuartRuAngelie Edrada-Ebel
Mar 9, 2020·Metabolomics : Official Journal of the Metabolomic Society·Keiron O'Shea, Biswapriya B Misra
May 23, 2020·Critical Reviews in Food Science and Nutrition·Shubo LiHongshun Yang
Aug 29, 2019·Cell Communication and Signaling : CCS·Giuseppina D'AlessandroCristina Limatola
Apr 2, 2020·Journal of the Endocrine Society·Riku KlénPirjo Nuutila
Jun 12, 2020·World Journal of Gastroenterology : WJG·Giulia NanniniLeonardo Tenori
Jun 28, 2020·Scientific Reports·Francesca AntonarosMaria Caracausi
Aug 17, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Sher AliAndersson Barison
Sep 24, 2020·International Journal of Molecular Sciences·Aneta BalcerczykGilles J P Rautureau
Aug 16, 2019·Expert Review of Molecular Diagnostics·Pro Kit CheungSze Chuen Cesar Wong
Mar 28, 2020·International Journal of Molecular Sciences·Maria Blanco-Formoso, Ramon A Alvarez-Puebla
Oct 11, 2020·Metabolites·Veronica GhiniClaudio Luchinat
Dec 24, 2020·Trends in Endocrinology and Metabolism : TEM·Decibel P ElpaPawel L Urban
Sep 3, 2019·Clinica Chimica Acta; International Journal of Clinical Chemistry·Yan-Ni WangYing-Yong Zhao
Feb 3, 2021·International Journal of Molecular Sciences·Caterina BernacchioniChiara Donati
Feb 6, 2021·Journal of Clinical Medicine·Ram B KhattriTerence E Ryan
Nov 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Alexandra A Crook, Robert Powers
Feb 11, 2021·Biomolecules·Fangrong ZhangTobias Madl
Feb 23, 2021·Journal of Proteome Research·Lenka MichálkováBohuš Bunganič
Feb 27, 2021·Progress in Nuclear Magnetic Resonance Spectroscopy·David A Snyder
Mar 7, 2021·Biomolecules·Eric Banan-Mwine DaliriDeog-Hwan Oh

❮ Previous
Next ❯

Methods Mentioned

BETA
NMR
sample collection
urine collections
PCA

Software Mentioned

NMR Suite
SPIDIA
JuiceScreener
FoodScreenerTM
MetaboAnalyst
DA
BAYESIL
OPLS
ASICS
BATMAN

Related Concepts

Related Feeds

Antiparasitics

Antiparasitics are medications which are indicated for the treatment of parasitic diseases. Discover the latest research on antiparasitics here.

Related Papers

Frontiers in Bioengineering and Biotechnology
Arnald AlonsoAntonio Julià
Annals of the New York Academy of Sciences
D Black
Annales de biologie clinique
Juan Manuel Chao De La BarcaGilles Simard
Cell Metabolism
Jeffrey R Idle, Frank J Gonzalez
© 2022 Meta ULC. All rights reserved