High-throughput miniaturized microfluidic microscopy with radially parallelized channel geometry

Analytical and Bioanalytical Chemistry
Veerendra Kalyan JagannadhSai Siva Gorthi

Abstract

In this article, we present a novel approach to throughput enhancement in miniaturized microfluidic microscopy systems. Using the presented approach, we demonstrate an inexpensive yet high-throughput analytical instrument. Using the high-throughput analytical instrument, we have been able to achieve about 125,880 cells per minute (more than one hundred and twenty five thousand cells per minute), even while employing cost-effective low frame rate cameras (120 fps). The throughput achieved here is a notable progression in the field of diagnostics as it enables rapid quantitative testing and analysis. We demonstrate the applicability of the instrument to point-of-care diagnostics, by performing blood cell counting. We report a comparative analysis between the counts (in cells per μl) obtained from our instrument, with that of a commercially available hematology analyzer.

References

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Citations

Aug 26, 2016·SLAS Technology·Akhil Chaturvedi, Sai Siva Gorthi
Jan 7, 2017·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·G GopakumarGorthi R K Sai Subrahmanyam
Jun 25, 2018·Analytical and Bioanalytical Chemistry·Michael Mayer, Antje J Baeumner
Jan 16, 2020·Micromachines·Gangadhar EluruSai Siva Gorthi
Sep 24, 2016·Optics Express·Veerendra Kalyan JagannadhSai Siva Gorthi

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