High throughput multiple locus variable number of tandem repeat analysis (MLVA) of Staphylococcus aureus from human, animal and food sources.

PloS One
Daniel SobralGilles Vergnaud

Abstract

Staphylococcus aureus is a major human pathogen, a relevant pathogen in veterinary medicine, and a major cause of food poisoning. Epidemiological investigation tools are needed to establish surveillance of S. aureus strains in humans, animals and food. In this study, we investigated 145 S. aureus isolates recovered from various animal species, disease conditions, food products and food poisoning events. Multiple Locus Variable Number of Tandem Repeat (VNTR) analysis (MLVA), known to be highly efficient for the genotyping of human S. aureus isolates, was used and shown to be equally well suited for the typing of animal S. aureus isolates. MLVA was improved by using sixteen VNTR loci amplified in two multiplex PCRs and analyzed by capillary electrophoresis ensuring a high throughput and high discriminatory power. The isolates were assigned to twelve known clonal complexes (CCs) and--a few singletons. Half of the test collection belonged to four CCs (CC9, CC97, CC133, CC398) previously described as mostly associated with animals. The remaining eight CCs (CC1, CC5, CC8, CC15, CC25, CC30, CC45, CC51), representing 46% of the animal isolates, are common in humans. Interestingly, isolates responsible for food poisoning show a CC distr...Continue Reading

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Citations

Mar 30, 2016·Systematic and Applied Microbiology·Sophia JohlerTom Grunert
Sep 10, 2015·Clinical Chemistry and Laboratory Medicine : CCLM·Dong-Sheng Lian, Shu-Jin Zhao
Jun 12, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Claire L RussellRosemary Grogono-Thomas
May 13, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yolande HauckChristine Pourcel
Feb 24, 2015·Vector Borne and Zoonotic Diseases·Haythem GharsaCarmen Torres
Dec 3, 2016·Journal of Medical Microbiology·Kavitha ShettigarThokur Sreepathy Murali
Nov 11, 2017·Frontiers in Microbiology·Karen K DittmannVirginie Oxaran
Oct 7, 2017·Transboundary and Emerging Diseases·P RainardJ R Middleton
Sep 2, 2016·Veterinary Dermatology·Stefan SchwarzKristina Kadlec

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Methods Mentioned

BETA
electrophoresis
PCRs
PCR
Assay
genotyping

Software Mentioned

GeneMapper
UPGMA
MLST
MLVA
BioNumerics
STAR

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