High throughput, parallel imaging and biomarker quantification of human spermatozoa by ImageStream flow cytometry

Systems Biology in Reproductive Medicine
Clayton BuckmanPeter Sutovsky

Abstract

Spermatid specific thioredoxin-3 protein (SPTRX-3) accumulates in the superfluous cytoplasm of defective human spermatozoa. Novel ImageStream technology combining flow cytometry with cell imaging was used for parallel quantification and visualization of SPTRX-3 protein in defective spermatozoa of five men from infertile couples. The majority of the SPTRX-3 containing cells were overwhelmingly spermatozoa with a variety of morphological defects, detectable in the ImageStream recorded images. Quantitative parameters of relative SPTRX-3 induced fluorescence measured by ImageStream correlated closely with conventional flow cytometric measurements of the same sample set and reflected the results of clinical semen evaluation. Image Stream quantification of SPTRX-3 combines and surpasses the informative value of both conventional flow cytometry and light microscopic semen evaluation. The observed patterns of the retention of SPTRX-3 in the sperm samples from infertility patients support the view that SPTRX3 is a biomarker of male infertility.

References

Jan 10, 2004·Antioxidants & Redox Signaling·Antonio Miranda-VizueteRichard Oko
Jun 2, 2004·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Thaddeus C GeorgePhilip J Morrissey
Jan 1, 2005·Theriogenology·Lindsay GillanW M C Maxwell
Feb 12, 2005·Human Reproduction·Monica MuratoriElisabetta Baldi
Dec 17, 2005·Advances in Clinical Chemistry·R F A WeberJ C Romijn
Sep 14, 2006·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·William E OrtynPhilip J Morrissey
Nov 23, 2006·Antioxidants & Redox Signaling·Christopher Horst Lillig, Arne Holmgren
Nov 23, 2006·Theriogenology·K W LovercampP Sutovsky
Jan 4, 2008·Nature·Shang-Zhong XuDavid J Beech
Apr 22, 2008·The Urologic Clinics of North America·Ashok AgarwalEdmund Sabanegh
Jun 24, 2008·Systems Biology in Reproductive Medicine·Sheena E M LewisJuan Alvarez
Nov 8, 2008·Nature Medicine·Martin M Matzuk, Dolores J Lamb
Dec 17, 2008·Reproductive Biomedicine Online·Nicolás GarridoMarcos Meseguer

❮ Previous
Next ❯

Citations

Jun 22, 2010·Asian Journal of Andrology·Wen-Bing ZhuLi-Qing Fan
Apr 12, 2011·Asian Journal of Andrology·Md Sharoare HossainHeriberto Rodriguez-Martinez
Nov 20, 2012·Bioanalysis·Ruth M Barnard
Aug 24, 2013·Reproduction in Domestic Animals = Zuchthygiene·A M Petrunkina, R A P Harrison
May 7, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Donald WlodkowicJonathan M Cooper
Mar 31, 2011·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·A M Petrunkina, R A P Harrison
Jul 16, 2010·Reproduction in Domestic Animals = Zuchthygiene·F Martínez-PastorP de Paz
Jul 16, 2015·Reproduction in Domestic Animals = Zuchthygiene·P Sutovsky
Jan 14, 2012·Systems Biology in Reproductive Medicine·Ester Anton, Stephen A Krawetz
Aug 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·C Ortega-FerrusolaP Martín-Muñoz
Sep 13, 2019·Reproduction in Domestic Animals = Zuchthygiene·Stuart MeyersAzarene Foutouhi
Dec 21, 2019·Reproductive Biology and Endocrinology : RB&E·Lukas DedJana Peknicova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.