High-throughput pharmacokinetic method: cassette dosing in mice associated with minuscule serial bleedings and LC/MS/MS analysis.

Analytica Chimica Acta
Takaho WatanabeBruce D Hammock

Abstract

A method for pharmacokinetic studies using cassette dosing associated with serial bleeding in mice is described. PK profiles of four soluble epoxide hydrolase inhibitors were determined following oral, subcutaneous or intraperitoneal administration individually or in cassette dosing. Parent analyses were performed on only 5 microL of whole blood from serial bleeds (up to 10 per animal), by LC/MS/MS. An accuracy (88-100%) and precision (<10% RSD) were observed, leading to reliable datum points for PK calculation. PK profiles, T(max), C(max) and half-life values after cassette dosing were similar to the individual PK results. This method dramatically increases speed of data collection while dramatically reducing cost and animal usage. The results presented here clearly indicate that this proposed method could be applicable to high-throughput PK studies.

References

May 17, 2003·Nature Reviews. Drug Discovery·Konrad H BleicherAlexander I Alanine
Jun 20, 2003·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Takaho WatanabeBruce D Hammock
Jul 10, 2003·Drug Discovery Today·Cédric MerlotDennis J Church
Dec 4, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·E TessierZ Zhu

❮ Previous
Next ❯

Citations

Apr 4, 2008·Cell Biology and Toxicology·Alan R ParrishBruce D Hammock
Sep 29, 2011·European Journal of Drug Metabolism and Pharmacokinetics·Vishwanath KurawattimathSandhya Mandlekar
Oct 24, 2007·Journal of Pharmaceutical Sciences·Joel W Proksch, Keith W Ward
Dec 3, 2014·Journal of Pharmaceutical Sciences·Ayahisa WatanabeYoshitaka Yamaguchi
Apr 3, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Hsing-Ju TsaiBruce D Hammock
Apr 19, 2007·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Wenri ZhangNabil J Alkayed
May 14, 2009·The American Journal of Pathology·Alexis N SimpkinsJohn D Imig
May 30, 2006·Analytical Biochemistry·Nicola M WolfBruce D Hammock
May 27, 2010·British Journal of Cancer·P WorkmanUNKNOWN Committee of the National Cancer Research Institute
Nov 15, 2006·Journal of Mass Spectrometry : JMS
Nov 1, 2008·The Journal of Biological Chemistry·Ahmed E EnayetAllahDavid F Grant
Mar 13, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Sean D Kodani, Bruce D Hammock
Jul 10, 2007·Journal of Medicinal Chemistry·Sung Hee HwangBruce D Hammock
Jun 17, 2014·Journal of Medicinal Chemistry·Wu YangPatrick Y S Lam
Nov 15, 2011·Bioorganic & Medicinal Chemistry Letters·Stevan PecicDonald W Landry
Dec 15, 2012·Bioorganic & Medicinal Chemistry Letters·Stevan PecicShi-Xian Deng

❮ Previous
Next ❯

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.