High throughput phenotyping of morpho-anatomical stem properties using X-ray computed tomography in sorghum

Plant Methods
Francisco E GomezWilliam L Rooney

Abstract

In bioenergy/forage sorghum, morpho-anatomical stem properties are major components affecting standability and juice yield. However, phenotyping these traits is low-throughput, and has been restricted by the lack of a high-throughput phenotyping platforms that can collect both morphological and anatomical stem properties. X-ray computed tomography (CT) offers a potential solution, but studies using this technology in plants have evaluated limited numbers of genotypes with limited throughput. Here we suggest that using a medical CT might overcome sample size limitations when higher resolution is not needed. Thus, the aim of this study was to develop a practical high-throughput phenotyping and image data processing pipeline that extracts stem morpho-anatomical traits faster, more efficiently and on a larger number of samples. A medical CT was used to image morpho-anatomical stem properties in sorghum. The platform and image analysis pipeline revealed extensive phenotypic variation for important morpho-anatomical traits in well-characterized sorghum genotypes at suitable repeatability rates. CT estimates were highly predictive of morphological traits and moderately predictive of anatomical traits. The image analysis pipeline also ...Continue Reading

References

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Citations

Sep 28, 2018·The Plant Journal : for Cell and Molecular Biology·Richard E BoylesStephen Kresovich
Mar 12, 2020·Plant Methods·Helin DutagaciDavid Rousseau
Apr 9, 2020·Plant Methods·Will H SeegmillerDaniel J Robertson
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Daoliang LiAkhter Muhammad
Apr 7, 2020·Journal of the Mechanical Behavior of Biomedical Materials·Seunghyun LeeMatt Pharr

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Methods Mentioned

BETA
imaging technology
X-ray
genotyping

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