High-throughput profiling of ion channel activity in primary human lymphocytes

Analytical Chemistry
Daniel J EstesMichael Mayer

Abstract

We present a high-throughput method to quantify the functional activity of potassium (K (+)) ion channels in primary human lymphocytes. This method is rapid, automated, specific (here for the voltage-gated Kv1.3 ion channel), and capable of measuring, in parallel, the electrical currents of over 200 individual lymphocytes isolated from freshly drawn blood. The statistics afforded by high-throughput measurements allowed direct comparison of Kv1.3 activity in different subsets of lymphocytes, including CD4 (+) and CD8 (+) T cells, gammadelta T cells, and B cells. High-throughput measurements made it possible to quantify the heterogeneous, functional response of Kv1.3 ion channel activity upon stimulation of CD4 (+) and CD8 (+) T cells with mitogen. These experiments enabled elucidation of time-courses of functional Kv1.3 activity upon stimulation as well as studies of the effects of the concentration of mitogenic antibodies on Kv1.3 levels. The results presented here suggest that Kv1.3 ion channel activity can be used as a functional activation marker in T cells and that it correlates to cell size and levels of a surface antigen, CD25. Moreover, this work presents an enabling methodology that can be applied widely, allowing high-...Continue Reading

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Citations

Nov 27, 2008·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Howard E GendelmanHuangui Xiong
Aug 13, 2013·Accounts of Chemical Research·Michael Mayer, Jerry Yang
Feb 9, 2016·Autoimmunity Reviews·Anantha Maharasi RamaKrishnan, Kavitha Sankaranarayanan
Jun 22, 2010·Current Opinion in Biotechnology·Sheereen MajdMichael Mayer
May 4, 2010·Chemistry and Physics of Lipids·Thuy Hien T NguyenPreston B Moore
Apr 1, 2008·Drug Discovery Today. Technologies
Aug 13, 2011·European Journal of Immunology·Mary C ReneerFrancesc Marti
Dec 25, 2012·The Journal of Asthma : Official Journal of the Association for the Care of Asthma·A Catalina Vélez-OrtegaFrancesc Marti
Oct 8, 2013·Biophysical Journal·Thuy Hien T NguyenPreston B Moore
Feb 23, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Erik C YuskoMichael Mayer
Apr 13, 2021·Frontiers in Pharmacology·Verena HofschröerAlbrecht Schwab

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