High-throughput profiling of the circulating proteome suggests sexually dimorphic corticosteroid signaling following ischemic stroke

Physiological Genomics
Grant C O'ConnellTaura L Barr

Abstract

Increasing evidence suggests that there are innate differences between sexes with respect to stroke pathophysiology; however, the molecular mechanisms underlying these differences remain unclear. In this investigation, we employed a shotgun approach to broadly profile sex-associated differences in the plasma proteomes of a small group of male ( n = 6) and female ( n = 4) ischemic stroke patients. Peripheral blood was sampled during the acute phase of care, and liquid chromatography electrospray ionization mass spectrometry was used to quantify plasma proteins. We observed widespread differences in plasma composition, as 77 out of 294 detected proteins were significantly differentially expressed between sexes. Corticosteroid-binding globulin (CBG), a negative acute-phase reactant that inversely regulates levels of bioactive free cortisol, was the most dramatically differentially regulated, exhibiting 16-fold higher abundance in plasma from women relative to men. Furthermore, functional annotation analysis revealed that the remaining differentially expressed proteins were significantly enriched for those involved in response to corticosteroid signaling. Plasma CBG levels were further examined in an additional group of male ( n = ...Continue Reading

References

Nov 1, 1992·Psychosomatic Medicine·C KirschbaumD Hellhammer
Jul 1, 1991·Stroke; a Journal of Cerebral Circulation·P A O'NeillD Bennett
Dec 1, 1990·Stroke; a Journal of Cerebral Circulation·H TohgiH Suzuki
Jul 4, 2003·The Clinical Journal of Pain·Christiane ZimmerStefan Lautenbacher
Jul 23, 2003·Neurology·Heather J FullertonS Claiborne Johnston
Nov 8, 2003·Stroke; a Journal of Cerebral Circulation·Chelsea S Kidwell, Steven Warach
Jan 27, 2007·Stroke; a Journal of Cerebral Circulation·Angel ChamorroAnna M Planas
Aug 30, 2008·Lancet Neurology·Mathew J ReevesLynda Lisabeth
Sep 24, 2008·Cerebrovascular Diseases·L Christine Turtzo, Louise D McCullough
Feb 13, 2009·Stroke; a Journal of Cerebral Circulation·Rodica E PetreaPhilip A Wolf
Feb 13, 2009·Stroke; a Journal of Cerebral Circulation·Peter AppelrosAndreas Terént
Jun 3, 2009·Nature Reviews. Endocrinology·George P Chrousos
Nov 4, 2010·Pediatric Research·Dino A GiussaniDavid S Gardner
May 10, 2012·JAMA : the Journal of the American Medical Association·Meytal Avgil TsadokLouise Pilote
Jun 6, 2012·Nature Reviews. Neurology·Ángel ChamorroRoland Veltkamp
Oct 4, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Roy A M HaastAmanda J Kiliaan
Sep 3, 2013·Experimental Neurology·Bharti ManwaniLouise D McCullough
Nov 28, 2013·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Glen C JicklingFrank R Sharp
Dec 11, 2013·Translational Stroke Research·Edward C Koellhoffer, Louise D McCullough
Jan 31, 2014·Journal of Neurology·Amanda Jayne BarughGillian Elizabeth Mead
Dec 18, 2014·Critical Care Medicine·Ben GibbisonStafford L Lightman
Jul 8, 2015·Journal of the American Heart Association·Christian DehlendorffTom Skyhøj Olsen
May 24, 2016·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·E J MeyerD J Torpy
Jul 2, 2016·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·James F ColbertAdit A Ginde
Dec 3, 2016·Nucleic Acids Research·UNKNOWN The UniProt Consortium
Feb 2, 2017·Stroke; a Journal of Cerebral Circulation·Fianne H SpaanderUNKNOWN Thrombolysis in Ischemic Stroke Patients Collaborators (TrISP)
Feb 6, 2017·Stroke; a Journal of Cerebral Circulation·Clemens LangUNKNOWN Austrian Stroke Registry Collaborators
Feb 6, 2017·Circulation Research·Valery L FeiginGeorge A Mensah
Nov 4, 2017·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Dannielle ZierathKyra J Becker

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Citations

Oct 22, 2021·Journal of Proteome Research·Eric W DeutschJochen M Schwenk
Nov 10, 2021·Thrombosis and Haemostasis·Natasha LetunicaVera Ignjatovic

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Datasets Mentioned

BETA
GM-109098

Methods Mentioned

BETA
ELISA
blood draw

Software Mentioned

R
Proteome Discoverer
Uniprot ID mapping
Proteome
topGO
Scaffold
Xcalaber

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