High-throughput purification of compound libraries

Drug Discovery Today
W C. RipkaJ Krakover

Abstract

Synthesis of combinatorial libraries by parallel synthesis, followed by high- throughput biological screening, is the new paradigm for drug discovery. Purity of these libraries is an important consideration to obtain high-quality assay data. Liquid-liquid extraction and solid-phase capture reagents are useful in special cases for small numbers of compounds. However, for libraries of a few thousand compounds, HPLC is a viable alternative. Beyond these numbers, factors such as solvent requirements, the number of fractions and tracking become prohibitive. Supercritical fluid chromatography has been successfully employed in automated purification instrumentation and is expected to be capable of purifying libraries of tens-of-thousands of compounds.

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Citations

Jul 27, 2001·Drug Discovery Today·J EverettN Terrett
Jan 16, 2002·Drug Discovery Today·David Cork, Nick Hird
Feb 13, 2004·Drug Discovery Today·András GuttmanJulia Khandurina
Jul 9, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Gitta SchlosserFerenc Hudecz
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Jul 19, 2006·Drug Discovery Today·Christoph M Huwe
May 23, 2006·Drug Discovery Today·Marcus Koppitz, Knut Eis
Jun 2, 2009·Journal of Biomolecular Screening·William P Janzen, Ioana G Popa-Burke
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Jul 2, 2002·Analytical Chemistry·Xueheng Cheng, Jill Hochlowski
Jul 2, 2002·Analytical Chemistry·Thomas L Chester, J David Pinkston
Oct 7, 2010·Journal of Combinatorial Chemistry·Harold N WellerPaul Lefebvre
Sep 12, 2006·Journal of Combinatorial Chemistry·Xu ZhangWolfgang K Goetzinger

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