High-throughput screening assays for SARS-CoV-2 drug development: Current status and future directions.

Drug Discovery Today
Tuan XuRuili Huang

Abstract

In response to the ongoing coronavirus disease 2019 (COVID-19) pandemic, a panel of assays has been developed and applied to screen collections of approved and investigational drugs for anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) activity in a quantitative high-throughput screening (qHTS) format. In this review, we applied data-driven approaches to evaluate the ability of each assay to identify potential anti-SARS-CoV-2 leads. Multitarget assays were found to show advantages in terms of accuracy and efficiency over single-target assays, whereas target-specific assays were more suitable for investigating compound mechanisms of action. Moreover, strict filtering with counter screens might be more detrimental than beneficial in identifying true positives. Thus, developing novel HTS assays acting simultaneously against multiple targets in the SARS-CoV-2 life cycle will benefit anti-COVID-19 drug discovery.

References

Apr 6, 2005·Trends in Pharmacological Sciences·Péter CsermelySándor Pongor
Jan 19, 2008·Molecular Cancer Therapeutics·Tao ZhangDuxin Sun
May 13, 2010·The Annals of Pharmacotherapy·Shelby L Corman, Rima A Mohammad
Feb 19, 2011·Current Chemical Genomics·Yuhong WangDac-Trung Nguyen
May 6, 2011·Environmental Health Perspectives·Ruili HuangChristopher P Austin
Jul 16, 2013·Drug Discovery Today·Wei ZhengJohn C McKew
Aug 11, 2016·Drug Discovery Today·Priya BawaViness Pillay
Jan 3, 2018·Biotechnology Advances·Michaela Rumlová, Tomáš Ruml
Jan 18, 2018·Clinical and Translational Medicine·Rona R RamsayMaria Laura Bolognesi
Apr 9, 2019·Assay and Drug Development Technologies·Emily M LeeWei Zheng
Feb 23, 2020·Drug Metabolism Reviews·Neha MaharaoJyotika Varshney
Mar 8, 2020·Journal of Autoimmunity·Yongshi YangChristopher Chang
Apr 11, 2020·The New England Journal of Medicine·Jonathan GreinTimothy Flanigan
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Kirill GorshkovWei Zheng
Jun 9, 2020·BioRxiv : the Preprint Server for Biology·Kyle R BrimacombeMin Shen
Jun 17, 2020·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Siqin FengShuyang Zhang
Jun 17, 2020·The Journal of Physical Chemistry Letters·Kaifu GaoGuo-Wei Wei
Jul 30, 2020·The Journal of Pharmacology and Experimental Therapeutics·Zeenat A ShyrWei Zheng
Oct 5, 2020·Signal Transduction and Targeted Therapy·Zhe-Rui ZhangBo Zhang
Oct 17, 2020·ACS Pharmacology & Translational Science·Wei ZhuWei Zheng
Oct 21, 2020·Science Translational Medicine·Barton F HaynesAnn Arvin
Nov 1, 2020·Journal of Clinical Pharmacy and Therapeutics·Havva KocayiğitAli Fuat Erdem
Dec 18, 2020·ACS Pharmacology & Translational Science·Catherine Z ChenGary R Whittaker
Dec 31, 2020·The New England Journal of Medicine·Mariana C Castells, Elizabeth J Phillips
Jan 4, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Nathalie WurtzBernard La Scola
Mar 13, 2021·Frontiers in Pharmacology·Catherine Z ChenWei Zheng

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Software Mentioned

ACE2
AlphaLISA
Spike
R package ‘ pheatmap ’

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