High-throughput screening methods for nitrilases

Applied Microbiology and Biotechnology
Ya-Ping XueYu-Guo Zheng

Abstract

Nitrilases have been widely acknowledged as important alternatives to chemical catalysts, as they have been proved to transform an immense variety of nitriles under mild conditions and often in a stereoselective or regioselective manner. In the discovery of new nitrilases to establish viable industrial processes, screening plays an important role in identifying which subset of candidates contains a nitrilase of interest from a collection of organisms, clone banks, or enzyme libraries. However, the traditional methods for evaluating the nitrilases are a time-consuming, laborious, and costly process and have been regarded as a bottleneck in developing these nitrilases as industrial biocatalysts. In the past few years, a number of high-throughput screening methods have been developed for rapid evaluation and identification of nitrilases. Here, we review the various methodologies developed for high-throughput screening of nitrilases and focus on their advantages and limitations.

References

Mar 29, 1990·Biochimica Et Biophysica Acta·M B ArnaoF García-Cánovas
Jun 17, 2000·Journal of Microbiological Methods·M R DaddC J Knowles
Oct 17, 2002·Applied Microbiology and Biotechnology·A BanerjeeU C Banerjee
Dec 31, 2002·Current Opinion in Structural Biology·Charles Brenner
Jan 29, 2003·Biotechnology and Applied Biochemistry·Anirban BanerjeeUttam Chand Banerjee
Sep 18, 2003·Journal of the American Chemical Society·Grace DeSantisMark J Burk
Mar 12, 2004·Journal of Ethnopharmacology·Ilhami GülçinMehmet Emin Büyükokuroglu
Apr 7, 2004·Applied and Environmental Microbiology·Dan E RobertsonJay M Short
Sep 11, 2004·Current Opinion in Biotechnology·Jean-Philippe Goddard, Jean-Louis Reymond
Dec 13, 2006·Biotechnology and Bioengineering·Shijun WuMark S Payne
Dec 29, 2006·Chemistry & Biodiversity·Ram SinghDiwan S Rawat
Aug 7, 2007·Applied and Environmental Microbiology·Qing ZhuYuguo Zheng
Sep 6, 2007·Biotechnology and Bioengineering·Shijun WuMark S Payne
Oct 25, 2007·Applied Microbiology and Biotechnology·Yu-Guo ZhengYin-Chu Shen
Dec 7, 2007·Journal of Biotechnology·Ludmila MartínkováVladimír Kren
Jan 31, 2009·IUBMB Life·Hans LeemhuisLubbert Dijkhuizen
Nov 19, 2009·Journal of Industrial Microbiology & Biotechnology·M SantoshkumarTimmanagouda B Karegoudar
Jan 20, 2010·Current Opinion in Chemical Biology·Ludmila Martínková, Vladimír Kren
Jan 5, 2012·Microbial Cell Factories·Giusy Manuela AdamoMarina Lotti
Mar 13, 2012·Analytical and Bioanalytical Chemistry·Matthew GrevingTrent Northen
Jun 27, 2013·Applied Microbiology and Biotechnology·Jin-Song GongZheng-Hong Xu
Jun 16, 2015·Industrial & Engineering Chemistry Research·Han XiaoHuimin Zhao

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Citations

Jun 20, 2020·Angewandte Chemie·Shuke WuUwe T Bornscheuer
Apr 7, 2018·Applied Microbiology and Biotechnology·Zhengming ZhuGuocheng Du
Jun 14, 2019·Bioprocess and Biosystems Engineering·Hai-Yan ZhouYu-Guo Zheng
Jul 9, 2017·Bioprocess and Biosystems Engineering·Ya-Ping XueYu-Guo Zheng
Oct 14, 2020·Critical Reviews in Biotechnology·Ji-Dong ShenYu-Guo Zheng

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