High-throughput screening of biocatalytic activity: applications in drug discovery

Current Opinion in Chemical Biology
R Anand Kumar, Douglas S Clark

Abstract

Enzymes catalyze a diverse set of reactions that propel life's processes and hence serve as valuable therapeutic targets. High-throughput screening methods have become essential for sifting through large chemical libraries in search of drug candidates, and several sensitive and reliable analytical techniques have been specifically adapted to high-throughput measurements of biocatalytic activity. High-throughput biocatalytic assay platforms thus enable rapid screening against enzymatic targets, and have vast potential to impact various stages of the drug discovery process, including lead identification and optimization, and ADME/Tox assessment. These advances are paving the way for the adoption of high-throughput biocatalytic assays as an indispensable tool for the pharmaceutical industry.

References

Mar 1, 2002·Biotechnology and Bioengineering·Chan B Park, Douglas S Clark
Dec 12, 2002·Journal of the American Chemical Society·Cleo M SalisburyJonathan A Ellman
Mar 4, 2003·Nature Reviews. Drug Discovery·H Mario GeysenRichard Wagner
Apr 3, 2003·Chembiochem : a European Journal of Chemical Biology·Grace Y J ChenShao Q Yao
Jul 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Dhaval N Gosalia, Scott L Diamond
Aug 30, 2003·Drug Discovery Today·Murray ColesHorst Kessler
Jan 6, 2004·Journal of Pharmaceutical Sciences·John H Ansede, Dhiren R Thakker
Jan 9, 2004·Journal of Biomolecular Screening·Duncan R GroebeUsha Warrior
Feb 6, 2004·American Journal of Physiology. Cell Physiology·A S Verkman
Mar 20, 2004·Science·Robert F Service
Mar 20, 2004·Science·Martin E M NobleLouise N Johnson
Mar 20, 2004·Science·Malcolm MacCoss, Thomas A Baillie
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Sriram KumaraswamyDavid Whitten
May 14, 2004·Journal of Biomolecular Screening·Kevin KupchoSaid A Goueli
May 29, 2004·Assay and Drug Development Technologies·James R BeasleyDavid A Dunn
May 29, 2004·Assay and Drug Development Technologies·Mitsunori Koresawa, Takayoshi Okabe
Jun 9, 2004·Current Opinion in Chemical Biology·Daniel B Kassel
Jan 15, 2005·Analytical Chemistry·Peter Babiak, Jean-Louis Reymond
Jan 20, 2005·Proceedings of the National Academy of Sciences of the United States of America·Moo-Yeal LeeDouglas S Clark
Feb 9, 2005·Proteomics·Philipp AngenendtJörn Glökler
Mar 3, 2005·Chembiochem : a European Journal of Chemical Biology·Oliver von Ahsen, Ulf Bömer
Mar 3, 2005·Chembiochem : a European Journal of Chemical Biology·Mike SchutkowskiUlf Reimer
Apr 6, 2005·Bioorganic & Medicinal Chemistry Letters·Mahesh UttamchandaniShao Q Yao
May 6, 2005·Assay and Drug Development Technologies·Haiching MaScott L Diamond
Jul 12, 2005·Current Opinion in Biotechnology·Janie S MerkelPaul F Predki
Sep 13, 2005·Journal of Combinatorial Chemistry·Marcus KoppitzMarion Wenz
Oct 6, 2005·Journal of Biochemistry and Molecular Biology·Dong-Sik ShinYoon-Sik Lee
Oct 15, 2005·Nature Reviews. Drug Discovery·Larry C Wienkers, Timothy G Heath
Nov 1, 2005·Analytical Chemistry·Kumiko Sakai-KatoNaoko Utsunomiya-Tate
Dec 15, 2005·Analytical Chemistry·Nicholas RupcichJohn D Brennan

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Citations

Feb 9, 2012·International Journal of Molecular Sciences·Paweł SzymańskiElżbieta Mikiciuk-Olasik
Dec 2, 2015·Expert Opinion on Drug Discovery·Changjiang You, Jacob Piehler
Jul 20, 2012·Biosensors & Bioelectronics·Elizabeth Schneider, Douglas S Clark
Oct 19, 2006·Current Opinion in Biotechnology·Moo-Yeal Lee, Jonathan S Dordick
Feb 18, 2015·Future Microbiology·Anand SrinivasanAnand K Ramasubramanian
Apr 4, 2017·Chemistry : a European Journal·Chloe WestleyRoyston Goodacre
Mar 29, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Fernando A Rojas-RuizVladimir V Kouznetsov
Mar 23, 2007·Electrophoresis·Jiri KonecnýZdenek Glatz
Jan 15, 2008·Chemistry, an Asian Journal·Daniel Kahakeaw, Manfred T Reetz
Apr 15, 2009·Current Protocols in Protein Science·Brian G Fox, Paul G Blommel
Feb 19, 2011·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V ShumiantsevaA I Archakov
May 16, 2015·Biomedit︠s︡inskai︠a︡ khimii︠a︡·V V ShumyantsevaA I Archakov
Oct 9, 2008·Journal of the American Chemical Society·Shin MizukamiKazuya Kikuchi
Apr 11, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Claire JeanquartierJohannes G Khinast
Jan 16, 2007·Analytical Chemistry·Linlin SunZhenxin Wang

❮ Previous
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