High throughput screening of CO2-tolerating microalgae using GasPak bags

Aquatic Biosystems
Zheng LiuFeng Chen

Abstract

Microalgae are diverse in terms of their speciation and function. More than 35,000 algal strains have been described, and thousands of algal cultures are maintained in different culture collection centers. The ability of CO2 uptake by microalgae varies dramatically among algal species. It becomes challenging to select suitable algal candidates that can proliferate under high CO2 concentration from a large collection of algal cultures. Here, we described a high throughput screening method to rapidly identify high CO2 affinity microalgae. The system integrates a CO2 mixer, GasPak bags and microplates. Microalgae on the microplates will be cultivated in GasPak bags charged with different CO2 concentrations. Using this method, we identified 17 algal strains whose growth rates were not influenced when the concentration of CO2 was increased from 2 to 20% (v/v). Most CO2 tolerant strains identified in this study were closely related to the species Scenedesmus and Chlorococcum. One of Scenedesmus strains (E7A) has been successfully tested in in the scale up photo bioreactors (500 L) bubbled with flue gas which contains 10-12% CO2. Our high throughput CO2 testing system provides a rapid and reliable way for identifying microalgal candid...Continue Reading

References

Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
May 17, 2008·Applied Microbiology and Biotechnology·Bei WangChristopher Q Lan
Dec 20, 2008·Applied Microbiology and Biotechnology·I DouskovaM Vitova
Dec 28, 2011·Marine Biotechnology·Vinitha EbenezerJang-Seu Ki

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