High throughput sequencing analysis of Trypanosoma brucei DRBD3/PTB1-bound mRNAs

Molecular and Biochemical Parasitology
Anish DasAntonio M Estévez

Abstract

Trypanosomes are early-branched eukaryotes that show an unusual dependence on post-transcriptional mechanisms to regulate gene expression. RNA-binding proteins are crucial in controlling mRNA fate in these organisms, but their RNA substrates remain largely unknown. Here we have analyzed on a global scale the mRNAs associated with the Trypanosoma brucei RNA-binding protein DRBD3/PTB1, by capturing ribonucleoprotein complexes using UV cross-linking and subsequent immunoprecipitation. DRBD3/PTB1 associates with many transcripts encoding ribosomal proteins and translation factors. Consequently, silencing of DRBD3/PTB1 expression altered the protein synthesis rate. DRBD3/PTB1 also binds to mRNAs encoding the enzymes required to obtain energy through the oxidation of proline to succinate. We hypothesize that DRBD3/PTB1 is a key player in RNA regulon-based gene control influencing protein synthesis in trypanosomes.

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Citations

Mar 17, 2016·World Journal of Biological Chemistry·Lysangela Ronalte Alves, Samuel Goldenberg
Aug 28, 2018·RNA Biology·Helisa Helena WippelLysangela Ronalte Alves
Sep 24, 2016·PLoS Pathogens·Víctor M Castillo-AcostaDolores González-Pacanowska
Jun 14, 2020·The Journal of Biological Chemistry·Larissa Melo do NascimentoChristine Clayton
May 28, 2019·Nucleic Acids Research·Anna TrenamanDavid Horn
Jan 11, 2017·Molecular and Biochemical Parasitology·Olivera CirovicTorsten Ochsenreiter

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