High-Throughput Sequencing Identifies Novel Viruses in Nectarine: Insights to the Etiology of Stem-Pitting Disease

Phytopathology
Dan Edward Veloso VillamorKen Eastwell

Abstract

Recent studies have shown the superiority of high-throughput sequencing (HTS) technology over many standard protocols for pathogen detection. HTS was initiated on fruit tree accessions from disparate sources to improve and advance virus-testing procedures. A virus with genomic features resembling most closely that of the recently described Nectarine stem-pitting-associated virus, putative member of genus Luteovirus, was found in three nectarine trees (Prunus persica cv. nectarina), each exhibiting stem-pitting symptoms on the woody cylinder above the graft union. In these samples, HTS also revealed the presence of a coinfecting virus with genome characteristics typical of members of the genus Marafivirus. The same marafivirus- and luteovirus-like viruses were detected in nonsymptomatic nectarine and peach selections, indicating only a loose relationship between these two viruses with nectarine stem-pitting disease symptoms. Two selections infected with each of these viruses had previously tested free of known virus or virus-like agents using the current biological, serological, and molecular tests employed at the Clean Plant Center Northwest. Overall, this study presents the characterization by HTS of novel marafivirus- and lut...Continue Reading

References

Jun 21, 2002·The Journal of General Virology·Leslie L DomierCleora J D'Arcy
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Feb 23, 2010·Virology·Beatrix CoetzeeJohan T Burger
Jun 1, 2012·The Journal of General Virology·Alan D RadfordNeil Hall
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Sep 13, 2016·Frontiers in Microbiology·Ahmed HadidiMarina Barba
Nov 7, 2016·Archives of Virology·D E V VillamorK C Eastwell
Feb 1, 2018·Scientific Reports·Yeonhwa JoWon Kyong Cho
Feb 26, 2019·Phytopathology·D E V VillamorI E Tzanetakis
Jan 30, 2018·Frontiers in Microbiology·Fan MuDaohong Jiang
Jul 7, 2020·EFSA Journal·UNKNOWN EFSA Panel on Plant Health (PLH)Luisa Rubino
Sep 27, 2018·Frontiers in Microbiology·Vicente PallásDelano James
Feb 28, 2020·Viruses·Wycliff M KinotiFiona E Constable
Jan 28, 2021·Viruses·Rachelle BesterHans J Maree
Feb 16, 2021·Frontiers in Microbiology·Muhammad Shafiq ShahidAbdullah M Al-Sadi
Apr 4, 2021·Viruses·Gajanan T MehetreBhim Pratap Singh

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