High-throughput sequencing of microRNAs in adenovirus type 3 infected human laryngeal epithelial cells.

Journal of Biomedicine & Biotechnology
Yuhua QiZuhong Lu

Abstract

Adenovirus infection can cause various illnesses depending on the infecting serotype, such as gastroenteritis, conjunctivitis, cystitis, and rash illness, but the infection mechanism is still unknown. MicroRNAs (miRNA) have been reported to play essential roles in cell proliferation, cell differentiation, and pathogenesis of human diseases including viral infections. We analyzed the miRNA expression profiles from adenovirus type 3 (AD3) infected Human laryngeal epithelial (Hep2) cells using a SOLiD deep sequencing. 492 precursor miRNAs were identified in the AD3 infected Hep2 cells, and 540 precursor miRNAs were identified in the control. A total of 44 miRNAs demonstrated high expression and 36 miRNAs showed lower expression in the AD3 infected cells than control. The biogenesis of miRNAs has been analyzed, and some of the SOLiD results were confirmed by Quantitative PCR analysis. The present studies may provide a useful clue for the biological function research into AD3 infection.

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Citations

Dec 21, 2011·Briefings in Functional Genomics·David J Studholme
Jul 1, 2011·Journal of Biomedicine & Biotechnology·Qian WuQinyu Ge
Feb 14, 2013·BMC Genomics·Zhi-Qiang DuJason W Ross
Apr 2, 2014·International Journal of Hematology·Kazuki OkuyamaAi Kotani
Mar 13, 2014·Ageing Research Reviews·Joseph M Dhahbi
Jul 25, 2012·Cellular and Molecular Life Sciences : CMLS·Leonidas BenetatosEvangelos Briasoulis
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Jul 8, 2011·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Anja P M Verhagen, Ger J M Pruijn
Mar 7, 2015·Virology·Hongxing ZhaoUlf Pettersson
Jul 5, 2016·Reviews in Medical Virology·Alireza TahamtanVahid Salimi
Jun 2, 2016·Scientific Reports·Claude Pasquier, Julien Gardès
Mar 31, 2017·Journal of Virology·Mitsuhiro MachitaniHiroyuki Mizuguchi
Dec 8, 2019·FEBS Letters·Hongxing ZhaoUlf Pettersson
Jun 10, 2015·Oncotarget·Berta Victoria MartinezMichal M Masternak
Jul 21, 2017·Frontiers in Oncology·Xavier Bofill-De RosCristina Fillat
Aug 5, 2017·Frontiers in Microbiology·Diogo Piedade, José M Azevedo-Pereira

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Methods Mentioned

BETA
FCS
PCR

Software Mentioned

SOLiD System Small RNA Analysis Pipeline Tool ( RNA2MAP )
SOLiD

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